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Solutions for manual RNA purification - (Nov/26/2007 )

Hello everybody,

I want ask about the method and concn of sodium citrate used for manual preparation of citrate-saturated phenol (pH 4.7) for RNA purification using citrate-saturated phenol (pH 4.7):chloroform:isoamyl alcohol (125:24:1) solution.

Thanks for ur interest.

-hamada_m-

QUOTE (hamada_m @ Nov 26 2007, 04:29 PM)
Hello everybody,

I want ask about the method and concn of sodium citrate used for manual preparation of citrate-saturated phenol (pH 4.7) for RNA purification using citrate-saturated phenol (pH 4.7):chloroform:isoamyl alcohol (125:24:1) solution.

Thanks for ur interest.

The solution is a 125:24:1 mixture of phenol:chloroform:isoamyl alcohol buffered with 42mM Sodium Citrate (pH 4.3-4.7). This formulation was found to significantly reduce contamination by chromosomal DNA. Chromosomal DNA contamination can result in nonspecific amplification during RT-PCR.
please visit
http://www.promega.com/faq/rnagents.html

-desertrose-

QUOTE (desertrose @ Nov 29 2007, 06:36 AM)
QUOTE (hamada_m @ Nov 26 2007, 04:29 PM)
Hello everybody,

I want ask about the method and concn of sodium citrate used for manual preparation of citrate-saturated phenol (pH 4.7) for RNA purification using citrate-saturated phenol (pH 4.7):chloroform:isoamyl alcohol (125:24:1) solution.

Thanks for ur interest.

The solution is a 125:24:1 mixture of phenol:chloroform:isoamyl alcohol buffered with 42mM Sodium Citrate (pH 4.3-4.7). This formulation was found to significantly reduce contamination by chromosomal DNA. Chromosomal DNA contamination can result in nonspecific amplification during RT-PCR.
please visit
http://www.promega.com/faq/rnagents.html

Thanks too much for ur invaluable help[color="#0000FF"][/color]

-hamada_m-