Tris or Hepes? - buffer choice (Jun/16/2008 )

Hi all!

What's the difference between Hepes pH=7.4 or Tris-Cl pH=7.4? I am making a lysis buffer, can I use one instead of the other?

Thanks,
BioPe

-BioPe-

QUOTE (BioPe @ Jun 16 2008, 02:55 AM)

Hi all!

What's the difference between Hepes pH=7.4 or Tris-Cl pH=7.4? I am making a lysis buffer, can I use one instead of the other?

Thanks,
BioPe

for lysis buffer, it should make no difference; I prefer HEPES as the pH is more stable over the time than that of Tris

-The Bearer-