Chromatofocuusing - How to make a pH gradiem (3-8) by using simple buffers. (Jun/05/2002 )

Can any body guide me how I can make a pH gradient 3-8 by simple buffers (not empholytes) chromatfocussing on anoin exchange column.
waiting to Thankyou,

Angelika Gorg (umlaut over the o), the princess of proteomics and 2-D gel electrophoresis has considerable information on creating IEF 1-D gels and how to make a 3-10 strip. Check her out online. CAs might be an unavoidable necessity.