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181. will site directed mutagenesis work for 8kb construct? - (reply: 4)
182. in-fusion cloning - (reply: 2)
183. M13 Hind and Sal digest - (reply: 2)
184. How to culture Top10 with pBI121 ? - (reply: 1)
185. Cre-ires-GFP - anyone have a construct with this? (reply: 4)
186. how long can I keep a ligation reaction going at 4 C? - (reply: 3)
187. Expression Vector sequencing problem - (reply: 6)
188. Trouble inserting PCR derived insert into vector - Trouble inserting PCR derived insert into vector (reply: 2)
189. Cant see ppt after ethanol precipitation?! - (reply: 3)
190. How to change Blunt End to sticky ? - (reply: 1)
191. homologous recombination in E coli - (reply: 10)
192. help with making ultra competent cell (Inoue method) - (reply: 3)
193. asking for kanamycin stock solution... - (reply: 4)
194. Primer extension and sequencing from total RNA - (reply: 1)
195. Plasmid Prep Problem : gDNA - bacterial genomic DNA contamination in a plasmid minipreparation (reply: 3)
196. cloning of a fluorecent PCR product - (reply: 1)
197. Can we store the gel slice after cutting out the band and before gel extraction? - (reply: 3)
198. Sequencing incorrect after digestion and ligation - (reply: 5)
199. Collagen bands in Western Blot - (reply: 2)
200. Cloning directionality and terminator availability - is lac z gene terminator is enough for my gene termination?? (reply: 1)
201. growth time of bacteria after transform - (reply: 2)
202. Any PBI121 Vector Information ? - Map, Sequence etc. (reply: 2)
203. how to check the insertion of a gene fragment into plasmid - (reply: 2)
204. Digesting ethanol precipitated DNA with AseI - (reply: 2)
205. Sequencing pDNA - (reply: 3)
206. Zero Blunt cloning - ligation problem? (reply: 3)
207. PCR to sequencing problems - (reply: 5)
208. Possible to add a linker to only one blunt end? - (reply: 4)
209. DNA gel to Test Ligation? - (reply: 2)
210. Problems during plasmid prep in cloning - (reply: 7)
182. in-fusion cloning - (reply: 2)
183. M13 Hind and Sal digest - (reply: 2)
184. How to culture Top10 with pBI121 ? - (reply: 1)
185. Cre-ires-GFP - anyone have a construct with this? (reply: 4)
186. how long can I keep a ligation reaction going at 4 C? - (reply: 3)
187. Expression Vector sequencing problem - (reply: 6)
188. Trouble inserting PCR derived insert into vector - Trouble inserting PCR derived insert into vector (reply: 2)
189. Cant see ppt after ethanol precipitation?! - (reply: 3)
190. How to change Blunt End to sticky ? - (reply: 1)
191. homologous recombination in E coli - (reply: 10)
192. help with making ultra competent cell (Inoue method) - (reply: 3)
193. asking for kanamycin stock solution... - (reply: 4)
194. Primer extension and sequencing from total RNA - (reply: 1)
195. Plasmid Prep Problem : gDNA - bacterial genomic DNA contamination in a plasmid minipreparation (reply: 3)
196. cloning of a fluorecent PCR product - (reply: 1)
197. Can we store the gel slice after cutting out the band and before gel extraction? - (reply: 3)
198. Sequencing incorrect after digestion and ligation - (reply: 5)
199. Collagen bands in Western Blot - (reply: 2)
200. Cloning directionality and terminator availability - is lac z gene terminator is enough for my gene termination?? (reply: 1)
201. growth time of bacteria after transform - (reply: 2)
202. Any PBI121 Vector Information ? - Map, Sequence etc. (reply: 2)
203. how to check the insertion of a gene fragment into plasmid - (reply: 2)
204. Digesting ethanol precipitated DNA with AseI - (reply: 2)
205. Sequencing pDNA - (reply: 3)
206. Zero Blunt cloning - ligation problem? (reply: 3)
207. PCR to sequencing problems - (reply: 5)
208. Possible to add a linker to only one blunt end? - (reply: 4)
209. DNA gel to Test Ligation? - (reply: 2)
210. Problems during plasmid prep in cloning - (reply: 7)