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241. dilute the ligation reaction for electroporation - (reply: 7)
242. digestion of pcr - (reply: 2)
243. Recombinant PCR - (reply: 1)
244. Plasmid lost during culture - (reply: 19)
245. pcr,ligation and transformation with RE SalI and XhoI - (reply: 4)
246. double digestion and ligation - (reply: 3)
247. cloning into the pBAD-GFPuv - (reply: 1)
248. Check the ligation product, but got two fragments with strange sizes on the gel - (reply: 7)
249. Restriction Enzyme Troubleshooting Help? - (reply: 2)
250. Ligation: time and temperature - (reply: 8)
251. PCR and competent cells - (reply: 3)
252. Suddenly no colonies, can cells 'go off' in -80? - (reply: 1)
253. How to prohibit BL21 Gold(DE3) plysS autolysis - (reply: 2)
254. Help restriction analysis of plasmid - (reply: 11)
255. Taq vs. T4 dna polymerse: difference? - (reply: 4)
256. Can I store plasmid in 4C refrigerator? - (reply: 11)
257. commercial TA cloning vector with Luciferase - cloning promoter sequences (reply: 2)
258. Cloning 1.5kB gene into lentivirus--I'm stumped - (reply: 2)
259. How to induce self-ligation of a vector? Self-Circularization? - (reply: 12)
260. Trying to cleave out unwanted nucleotide sequence in an insert. - (reply: 1)
261. Is it possible to use a PCR cleaning kit to clean a band from a TAE-Agarose gel? - (reply: 1)
262. How many bases should be added to end of enzyme in PCR for effective cutting - (reply: 8)
263. clear culture, but PCR positive - (reply: 4)
264. Many problems in genomic library construction - try to construction genomic library (reply: 3)
265. phophorylating PCR product after CIP - (reply: 2)
266. ligation - very small insert - problems during ligation (reply: 11)
267. How to find DNA sequence upstream of gene? - (reply: 3)
268. Multimerization after restriction digestion - (reply: 9)
269. low concentartion of DNA after miniprep - (reply: 6)
270. pcr template amount - (reply: 2)
242. digestion of pcr - (reply: 2)
243. Recombinant PCR - (reply: 1)
244. Plasmid lost during culture - (reply: 19)
245. pcr,ligation and transformation with RE SalI and XhoI - (reply: 4)
246. double digestion and ligation - (reply: 3)
247. cloning into the pBAD-GFPuv - (reply: 1)
248. Check the ligation product, but got two fragments with strange sizes on the gel - (reply: 7)
249. Restriction Enzyme Troubleshooting Help? - (reply: 2)
250. Ligation: time and temperature - (reply: 8)
251. PCR and competent cells - (reply: 3)
252. Suddenly no colonies, can cells 'go off' in -80? - (reply: 1)
253. How to prohibit BL21 Gold(DE3) plysS autolysis - (reply: 2)
254. Help restriction analysis of plasmid - (reply: 11)
255. Taq vs. T4 dna polymerse: difference? - (reply: 4)
256. Can I store plasmid in 4C refrigerator? - (reply: 11)
257. commercial TA cloning vector with Luciferase - cloning promoter sequences (reply: 2)
258. Cloning 1.5kB gene into lentivirus--I'm stumped - (reply: 2)
259. How to induce self-ligation of a vector? Self-Circularization? - (reply: 12)
260. Trying to cleave out unwanted nucleotide sequence in an insert. - (reply: 1)
261. Is it possible to use a PCR cleaning kit to clean a band from a TAE-Agarose gel? - (reply: 1)
262. How many bases should be added to end of enzyme in PCR for effective cutting - (reply: 8)
263. clear culture, but PCR positive - (reply: 4)
264. Many problems in genomic library construction - try to construction genomic library (reply: 3)
265. phophorylating PCR product after CIP - (reply: 2)
266. ligation - very small insert - problems during ligation (reply: 11)
267. How to find DNA sequence upstream of gene? - (reply: 3)
268. Multimerization after restriction digestion - (reply: 9)
269. low concentartion of DNA after miniprep - (reply: 6)
270. pcr template amount - (reply: 2)