|
331. too many cloning false positives - (reply: 5)
332. terrible clonning - (reply: 1)
333. mRNA vs ds cDNA - Size differenses between mRNA and ds cDNA (reply: 3)
334. how to do double digestion with EcoRI and BamHI - (reply: 9)
335. long insert ligation - (reply: 5)
336. How about vector sequence in cloning? - (reply: 9)
337. how to tell the transformation result? - (reply: 4)
338. colonies not growing on plates, but in culture - (reply: 1)
339. digested run slower on gel? - (reply: 6)
340. NEB restriction enzyme BssS1 - digest (reply: 1)
341. finding start and stop codon in an insert - (reply: 2)
342. nucleotide mutations introduced during transformation - (reply: 3)
343. do you put two pairs primers in one tube when you do site mutations? - (reply: 2)
344. GAPDH primer sequence - cloning of huGAPDH gene (reply: 2)
345. RT-PCR cloning: oligo dT-primed cDNA synthesis or anchored? - (reply: 1)
346. problems with restriction digestion - (reply: 7)
347. Big fragment ligation - (reply: 6)
348. How to know the sequence is in frame - (reply: 4)
349. cloning in pNL4-3 - (reply: 1)
350. ViraQuest Adeno map - (reply: 1)
351. Flag sequence - yeah I know, stupid question :D (reply: 3)
352. ligating 5 fragments together - how much the success possibility (reply: 11)
353. "read -through" transcription - (reply: 1)
354. Medium for electroporating HEK 293 T-cells - (reply: 2)
355. DH5 alpha methylation profile - (reply: 8)
356. Do you discard phosphatase before ligation? - (reply: 6)
357. Electroporation Lactic acid Bacteria - Problems establishment/reproducibility (reply: 1)
358. vector: Klenow + CIP - do they work together?! (reply: 1)
359. Adding A overhangs - (reply: 3)
360. Problems by using pcDNA 3.1/Myc-His(+) B - (reply: 2)
332. terrible clonning - (reply: 1)
333. mRNA vs ds cDNA - Size differenses between mRNA and ds cDNA (reply: 3)
334. how to do double digestion with EcoRI and BamHI - (reply: 9)
335. long insert ligation - (reply: 5)
336. How about vector sequence in cloning? - (reply: 9)
337. how to tell the transformation result? - (reply: 4)
338. colonies not growing on plates, but in culture - (reply: 1)
339. digested run slower on gel? - (reply: 6)
340. NEB restriction enzyme BssS1 - digest (reply: 1)
341. finding start and stop codon in an insert - (reply: 2)
342. nucleotide mutations introduced during transformation - (reply: 3)
343. do you put two pairs primers in one tube when you do site mutations? - (reply: 2)
344. GAPDH primer sequence - cloning of huGAPDH gene (reply: 2)
345. RT-PCR cloning: oligo dT-primed cDNA synthesis or anchored? - (reply: 1)
346. problems with restriction digestion - (reply: 7)
347. Big fragment ligation - (reply: 6)
348. How to know the sequence is in frame - (reply: 4)
349. cloning in pNL4-3 - (reply: 1)
350. ViraQuest Adeno map - (reply: 1)
351. Flag sequence - yeah I know, stupid question :D (reply: 3)
352. ligating 5 fragments together - how much the success possibility (reply: 11)
353. "read -through" transcription - (reply: 1)
354. Medium for electroporating HEK 293 T-cells - (reply: 2)
355. DH5 alpha methylation profile - (reply: 8)
356. Do you discard phosphatase before ligation? - (reply: 6)
357. Electroporation Lactic acid Bacteria - Problems establishment/reproducibility (reply: 1)
358. vector: Klenow + CIP - do they work together?! (reply: 1)
359. Adding A overhangs - (reply: 3)
360. Problems by using pcDNA 3.1/Myc-His(+) B - (reply: 2)