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571. Help -> Failed Transformations - (reply: 6)
572. Isolation of total RNA from different tissues - efficiency? - (reply: 2)
573. Problems with restriction enzyme digest. - (reply: 2)
574. PCR screening of colonies - (reply: 3)
575. Stable Clones protocol - (reply: 2)
576. Getting Vaccinia topoisomerase I to nick and covalently bind plasmid DNA - (reply: 2)
577. problems with mutant PCR - problems with mutant PCR (reply: 1)
578. cloning of 1 kb fragment in 14 kb vector - (reply: 3)
579. Problem with E.coli growing in LB - unexpected growth (reply: 1)
580. Colony PCR running as smears - (reply: 1)
581. adapter liagation into a single cut plasmid? - (reply: 4)
582. Restriction digested plasmid - (reply: 2)
583. Cloning small DNA fragments. Bacteria are not growing - (reply: 5)
584. cloning help about pBudCE4.1 - (reply: 1)
585. cloning of a big Plasmid - (reply: 3)
586. Partial Digestion and Religation - Partial Digestion to eliminate a restriction site (reply: 1)
587. Loss of insert after cloning! - (reply: 3)
588. cloning problem - (reply: 6)
589. Blunt Cloning Large Insert/Smaller Vector - (reply: 9)
590. Ligation with T4 DNA Ligase - (reply: 9)
591. PCR cloning: neg self-ligation control, many colonies but no insert - (reply: 3)
592. Cloning Related Problem - Cloning in pBSK (reply: 1)
593. Subtractive cDNA library - (reply: 4)
594. Problems with directional cloning using BsmBI and Xba I - (reply: 6)
595. Cloning trouble......help! - (reply: 4)
596. cloning problem of big inserts - (reply: 4)
597. how the multiple cloning site in cloning vector is create? - (reply: 4)
598. plasmid problem - (reply: 3)
599. 3 molar insert excess - how imp. is it for the ligation rxn (reply: 2)
600. Colony PCR - (reply: 8)
572. Isolation of total RNA from different tissues - efficiency? - (reply: 2)
573. Problems with restriction enzyme digest. - (reply: 2)
574. PCR screening of colonies - (reply: 3)
575. Stable Clones protocol - (reply: 2)
576. Getting Vaccinia topoisomerase I to nick and covalently bind plasmid DNA - (reply: 2)
577. problems with mutant PCR - problems with mutant PCR (reply: 1)
578. cloning of 1 kb fragment in 14 kb vector - (reply: 3)
579. Problem with E.coli growing in LB - unexpected growth (reply: 1)
580. Colony PCR running as smears - (reply: 1)
581. adapter liagation into a single cut plasmid? - (reply: 4)
582. Restriction digested plasmid - (reply: 2)
583. Cloning small DNA fragments. Bacteria are not growing - (reply: 5)
584. cloning help about pBudCE4.1 - (reply: 1)
585. cloning of a big Plasmid - (reply: 3)
586. Partial Digestion and Religation - Partial Digestion to eliminate a restriction site (reply: 1)
587. Loss of insert after cloning! - (reply: 3)
588. cloning problem - (reply: 6)
589. Blunt Cloning Large Insert/Smaller Vector - (reply: 9)
590. Ligation with T4 DNA Ligase - (reply: 9)
591. PCR cloning: neg self-ligation control, many colonies but no insert - (reply: 3)
592. Cloning Related Problem - Cloning in pBSK (reply: 1)
593. Subtractive cDNA library - (reply: 4)
594. Problems with directional cloning using BsmBI and Xba I - (reply: 6)
595. Cloning trouble......help! - (reply: 4)
596. cloning problem of big inserts - (reply: 4)
597. how the multiple cloning site in cloning vector is create? - (reply: 4)
598. plasmid problem - (reply: 3)
599. 3 molar insert excess - how imp. is it for the ligation rxn (reply: 2)
600. Colony PCR - (reply: 8)