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541. colony PCR screening, drove my crazy! need ur suggestion - (reply: 2)
542. Gateway cloning with 3 entry vectors - (reply: 4)
543. gel elution problem - (reply: 1)
544. ligation problem - (reply: 5)
545. Utter failure of directional cloning - need help - (reply: 5)
546. ligation - (reply: 5)
547. Problem with TA cloning - (reply: 1)
548. choosing competent cells - (reply: 5)
549. Cloning using adaptors-->converting RE site - (reply: 4)
550. primer design for site-directed mutagenesis - (reply: 3)
551. how to ensure that insert orientation is correct in TA cloning - (reply: 6)
552. using nanodrop to measure PCR product - (reply: 3)
553. I added 2.5 times as much LB to TSS (Transformation and Storage Buffer) - What would happen? (reply: 1)
554. Best compotent cells - (reply: 5)
555. how to sequence 1500+ bp fragment? - (reply: 6)
556. Culturing in 50mL conical tubes? - (reply: 6)
557. Contamination of DH5 alpha stocks? - Colonies produced with negative control (reply: 2)
558. Separate cutting plasmid from uncut? - (reply: 4)
559. plasmid DNA purification - which kit should I buy? (reply: 7)
560. Why acetylated BSA? - (reply: 1)
561. Change pBI121 promoter - (reply: 11)
562. how to switch to a gene in a vector using PCR or restriction enzymes? - gene is in pIVS2 vector (reply: 3)
563. pEBG vector...Where is it? - Is it possible to purchase this vector? (reply: 3)
564. Restriction Enzyme Binding - (reply: 3)
565. BAC vecor cloning - (reply: 3)
566. NotI / EcoRI digest - (reply: 5)
567. plasmid prep question - thick white material? (reply: 11)
568. help finding eukaryote expression vector - (reply: 2)
569. Verifying Restriction sites generated in PCR of insert for subcloning - No colonies in transformation!Ligation issues?Insert issue? (reply: 4)
570. Clontech's InFusion product - (reply: 1)
542. Gateway cloning with 3 entry vectors - (reply: 4)
543. gel elution problem - (reply: 1)
544. ligation problem - (reply: 5)
545. Utter failure of directional cloning - need help - (reply: 5)
546. ligation - (reply: 5)
547. Problem with TA cloning - (reply: 1)
548. choosing competent cells - (reply: 5)
549. Cloning using adaptors-->converting RE site - (reply: 4)
550. primer design for site-directed mutagenesis - (reply: 3)
551. how to ensure that insert orientation is correct in TA cloning - (reply: 6)
552. using nanodrop to measure PCR product - (reply: 3)
553. I added 2.5 times as much LB to TSS (Transformation and Storage Buffer) - What would happen? (reply: 1)
554. Best compotent cells - (reply: 5)
555. how to sequence 1500+ bp fragment? - (reply: 6)
556. Culturing in 50mL conical tubes? - (reply: 6)
557. Contamination of DH5 alpha stocks? - Colonies produced with negative control (reply: 2)
558. Separate cutting plasmid from uncut? - (reply: 4)
559. plasmid DNA purification - which kit should I buy? (reply: 7)
560. Why acetylated BSA? - (reply: 1)
561. Change pBI121 promoter - (reply: 11)
562. how to switch to a gene in a vector using PCR or restriction enzymes? - gene is in pIVS2 vector (reply: 3)
563. pEBG vector...Where is it? - Is it possible to purchase this vector? (reply: 3)
564. Restriction Enzyme Binding - (reply: 3)
565. BAC vecor cloning - (reply: 3)
566. NotI / EcoRI digest - (reply: 5)
567. plasmid prep question - thick white material? (reply: 11)
568. help finding eukaryote expression vector - (reply: 2)
569. Verifying Restriction sites generated in PCR of insert for subcloning - No colonies in transformation!Ligation issues?Insert issue? (reply: 4)
570. Clontech's InFusion product - (reply: 1)