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1441. RE Double Digest XhoI/NdeI TA Vector - (reply: 2)
1442. EcoRI problem - (reply: 4)
1443. Too many colonies - (reply: 8)
1444. Cloning primer-dimer fragments - (reply: 2)
1445. Xba I and subcloning - (reply: 10)
1446. gel purification of the vector... - is it good enough for ligation? (reply: 7)
1447. DNA amount for protein expression - protein His-tag (reply: 1)
1448. insert is being kicked out during cell growth - (reply: 3)
1449. PCR or digestions for clone screen? - (reply: 5)
1450. Help! Where Can i get the pShuttle of Clontech? - (reply: 1)
1451. decontamination of electrophoresis chambers... - what solution should be used? (reply: 2)
1452. pDNR vector - sequencing problems (reply: 1)
1453. minimal promoter - (reply: 4)
1454. stange cloning results - pBluescript seeming to double up (reply: 6)
1455. Amplification of specific genes from cDNA - (reply: 3)
1456. Unexpected antibiotics resistance - (reply: 4)
1457. Do you kill the ligation product? - (reply: 9)
1458. How to purify PCR product? - (reply: 3)
1459. How to tell if competent cells or SOC are contaminated? - (reply: 14)
1460. what competent cells should I use to clone big size DNA - (reply: 8)
1461. not able to ligate PCR product into a vector... - what do you suggest? (reply: 2)
1462. Incubation of transformants - (reply: 5)
1463. Forgot cDNA at Room Temperature ?! - is it still usable ? (reply: 3)
1464. purification of digested plasmids - (reply: 4)
1465. RNA contamination w/ ethanol - (reply: 18)
1466. Digestion and ligation problem - Insertion of 4Kb DNA fragment into a 10Kb vector (reply: 2)
1467. my insert is there by PCR check but no band after digestion... - vector not digested because of contamination? (reply: 3)
1468. Blunt PCR cloning not working - (reply: 2)
1469. Another Problematic Ligation - 110bp ligation into 2.5kb vector (reply: 7)
1470. pBABE plasmid - (reply: 3)
1442. EcoRI problem - (reply: 4)
1443. Too many colonies - (reply: 8)
1444. Cloning primer-dimer fragments - (reply: 2)
1445. Xba I and subcloning - (reply: 10)
1446. gel purification of the vector... - is it good enough for ligation? (reply: 7)
1447. DNA amount for protein expression - protein His-tag (reply: 1)
1448. insert is being kicked out during cell growth - (reply: 3)
1449. PCR or digestions for clone screen? - (reply: 5)
1450. Help! Where Can i get the pShuttle of Clontech? - (reply: 1)
1451. decontamination of electrophoresis chambers... - what solution should be used? (reply: 2)
1452. pDNR vector - sequencing problems (reply: 1)
1453. minimal promoter - (reply: 4)
1454. stange cloning results - pBluescript seeming to double up (reply: 6)
1455. Amplification of specific genes from cDNA - (reply: 3)
1456. Unexpected antibiotics resistance - (reply: 4)
1457. Do you kill the ligation product? - (reply: 9)
1458. How to purify PCR product? - (reply: 3)
1459. How to tell if competent cells or SOC are contaminated? - (reply: 14)
1460. what competent cells should I use to clone big size DNA - (reply: 8)
1461. not able to ligate PCR product into a vector... - what do you suggest? (reply: 2)
1462. Incubation of transformants - (reply: 5)
1463. Forgot cDNA at Room Temperature ?! - is it still usable ? (reply: 3)
1464. purification of digested plasmids - (reply: 4)
1465. RNA contamination w/ ethanol - (reply: 18)
1466. Digestion and ligation problem - Insertion of 4Kb DNA fragment into a 10Kb vector (reply: 2)
1467. my insert is there by PCR check but no band after digestion... - vector not digested because of contamination? (reply: 3)
1468. Blunt PCR cloning not working - (reply: 2)
1469. Another Problematic Ligation - 110bp ligation into 2.5kb vector (reply: 7)
1470. pBABE plasmid - (reply: 3)