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361. Rapid growth of transformed DH5 alpha cells - (reply: 4)
362. Sal I cloning problems - (reply: 13)
363. Is it possible to loose the insert? - (reply: 2)
364. problem with restriction digest - I have an insert, but no vector.... (reply: 5)
365. Freezing transformations? - (reply: 1)
366. Restriciton digest protocol for adjacent sites? - (reply: 7)
367. RE incubtation time - (reply: 5)
368. Ligation problem with PCR product cloning - (reply: 9)
369. point mutation - (reply: 8)
370. PCR cloning-primer design and ligation - (reply: 20)
371. For double sequential digest: which enzyme first? - (reply: 7)
372. problems in clone confirmation - (reply: 3)
373. Electroporation and arcing - (reply: 4)
374. large fragment cloning problem! Help needed - (reply: 2)
375. Annealing plasmid DNA - (reply: 3)
376. Differential display..... - (reply: 1)
377. Cloning trouble - (reply: 7)
378. Problems with double digestion - (reply: 1)
379. cloning - (reply: 2)
380. SOS: DNA purity problems - Low A260/230 of gel extracted DNA samples (reply: 4)
381. how to compare my DNA sequence with NCBI sequence? - (reply: 1)
382. Replacing EGFP with RFP..but no expression! - (reply: 10)
383. Unable to cut amplified fragments in promoter construct - (reply: 5)
384. why i couldn't get result for my northern.help! - (reply: 3)
385. Fast way to get plasmid for PCR - (reply: 2)
386. Problems after site directed mutagenesis - (reply: 5)
387. restriction enzyme site with primers - (reply: 4)
388. PCR product cloning - (reply: 6)
389. sequencing problem - (reply: 7)
390. PCR failure. GC problem? - (reply: 7)
362. Sal I cloning problems - (reply: 13)
363. Is it possible to loose the insert? - (reply: 2)
364. problem with restriction digest - I have an insert, but no vector.... (reply: 5)
365. Freezing transformations? - (reply: 1)
366. Restriciton digest protocol for adjacent sites? - (reply: 7)
367. RE incubtation time - (reply: 5)
368. Ligation problem with PCR product cloning - (reply: 9)
369. point mutation - (reply: 8)
370. PCR cloning-primer design and ligation - (reply: 20)
371. For double sequential digest: which enzyme first? - (reply: 7)
372. problems in clone confirmation - (reply: 3)
373. Electroporation and arcing - (reply: 4)
374. large fragment cloning problem! Help needed - (reply: 2)
375. Annealing plasmid DNA - (reply: 3)
376. Differential display..... - (reply: 1)
377. Cloning trouble - (reply: 7)
378. Problems with double digestion - (reply: 1)
379. cloning - (reply: 2)
380. SOS: DNA purity problems - Low A260/230 of gel extracted DNA samples (reply: 4)
381. how to compare my DNA sequence with NCBI sequence? - (reply: 1)
382. Replacing EGFP with RFP..but no expression! - (reply: 10)
383. Unable to cut amplified fragments in promoter construct - (reply: 5)
384. why i couldn't get result for my northern.help! - (reply: 3)
385. Fast way to get plasmid for PCR - (reply: 2)
386. Problems after site directed mutagenesis - (reply: 5)
387. restriction enzyme site with primers - (reply: 4)
388. PCR product cloning - (reply: 6)
389. sequencing problem - (reply: 7)
390. PCR failure. GC problem? - (reply: 7)