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631. SURE E. coli strain - How make competents cells? (reply: 4)
632. Is this genomic DNA? - or plasmid? (reply: 11)
633. cDNA library insert-vector ratio - (reply: 1)
634. Cloning/Miniprep Blues - High molecular weight bands iand no plasmid n my miniprep (reply: 3)
635. Ligation - (reply: 5)
636. site after blunting - (reply: 1)
637. Cloning w/pCR4 TOPO vector - problems obtaining clones (reply: 1)
638. blunt ending the vector followed by the ligation - (reply: 1)
639. Vector for expressing proteins to be secreted - (reply: 3)
640. Vector for producing Fc fusion proteins - (reply: 1)
641. Multiple fragment ligation - (reply: 7)
642. Kozak sequence between GST and gene of my interest - There is a kozak sequence between GST and my gene. Does it hurt? (reply: 1)
643. Colony PCR - (reply: 11)
644. primer dissolved in wrong buffer (1M Tris HCl) - what to do now?? (reply: 7)
645. HELP: Ligation Problems - (reply: 10)
646. three-point ligation - (reply: 6)
647. Stability of cut DNA - (reply: 8)
648. problem with transformed plasmid's size - (reply: 10)
649. Can't ligate 2 linear fragments with cohesive ends - (reply: 5)
650. pBJ5 sequence - (reply: 1)
651. Ligation of pBBR with PCR fragments doesn't work - (reply: 3)
652. How to get rid of satellite colonies? - (reply: 17)
653. NsiI digestion problems - NsiI is not working properly? (reply: 1)
654. Confused about cre-lox recombination system - Why is CreatorTM DNA cloning better over other cre-lox systems? (reply: 1)
655. kozak sequence has to be in frame? - (reply: 9)
656. does the high pH of milli Q water will affect my ligation? - (reply: 8)
657. Dilute re-ligation - deletion construct (reply: 1)
658. Weird thing: Cml resistant S. aureus strain (RN4220) - (reply: 1)
659. Problems with cloning - (reply: 4)
660. Issues with pcDNA3.1Directional cloning - (reply: 2)
632. Is this genomic DNA? - or plasmid? (reply: 11)
633. cDNA library insert-vector ratio - (reply: 1)
634. Cloning/Miniprep Blues - High molecular weight bands iand no plasmid n my miniprep (reply: 3)
635. Ligation - (reply: 5)
636. site after blunting - (reply: 1)
637. Cloning w/pCR4 TOPO vector - problems obtaining clones (reply: 1)
638. blunt ending the vector followed by the ligation - (reply: 1)
639. Vector for expressing proteins to be secreted - (reply: 3)
640. Vector for producing Fc fusion proteins - (reply: 1)
641. Multiple fragment ligation - (reply: 7)
642. Kozak sequence between GST and gene of my interest - There is a kozak sequence between GST and my gene. Does it hurt? (reply: 1)
643. Colony PCR - (reply: 11)
644. primer dissolved in wrong buffer (1M Tris HCl) - what to do now?? (reply: 7)
645. HELP: Ligation Problems - (reply: 10)
646. three-point ligation - (reply: 6)
647. Stability of cut DNA - (reply: 8)
648. problem with transformed plasmid's size - (reply: 10)
649. Can't ligate 2 linear fragments with cohesive ends - (reply: 5)
650. pBJ5 sequence - (reply: 1)
651. Ligation of pBBR with PCR fragments doesn't work - (reply: 3)
652. How to get rid of satellite colonies? - (reply: 17)
653. NsiI digestion problems - NsiI is not working properly? (reply: 1)
654. Confused about cre-lox recombination system - Why is CreatorTM DNA cloning better over other cre-lox systems? (reply: 1)
655. kozak sequence has to be in frame? - (reply: 9)
656. does the high pH of milli Q water will affect my ligation? - (reply: 8)
657. Dilute re-ligation - deletion construct (reply: 1)
658. Weird thing: Cml resistant S. aureus strain (RN4220) - (reply: 1)
659. Problems with cloning - (reply: 4)
660. Issues with pcDNA3.1Directional cloning - (reply: 2)