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601. STRATEGENE hybrizap pADGAL4-2.1 - pADGAL4-2.1 (reply: 1)
602. Calculation of MW fusion protein - (reply: 1)
603. Double digestion - (reply: 13)
604. genome walking - (reply: 1)
605. PCR OF HIV genome - (reply: 1)
606. Transformation - (reply: 5)
607. Is time of ligation insert and vector crucial? - (reply: 4)
608. High Throughput transformation - (reply: 5)
609. Retrieve dried vector - (reply: 2)
610. help for no E coli. clone after transforming - (reply: 5)
611. CTAB - (reply: 1)
612. SURE E. coli strain - How make competents cells? (reply: 4)
613. Is this genomic DNA? - or plasmid? (reply: 11)
614. cDNA library insert-vector ratio - (reply: 1)
615. Cloning/Miniprep Blues - High molecular weight bands iand no plasmid n my miniprep (reply: 3)
616. Ligation - (reply: 5)
617. site after blunting - (reply: 1)
618. Cloning w/pCR4 TOPO vector - problems obtaining clones (reply: 1)
619. blunt ending the vector followed by the ligation - (reply: 1)
620. Vector for expressing proteins to be secreted - (reply: 3)
621. Vector for producing Fc fusion proteins - (reply: 1)
622. Multiple fragment ligation - (reply: 7)
623. Kozak sequence between GST and gene of my interest - There is a kozak sequence between GST and my gene. Does it hurt? (reply: 1)
624. Colony PCR - (reply: 11)
625. primer dissolved in wrong buffer (1M Tris HCl) - what to do now?? (reply: 7)
626. HELP: Ligation Problems - (reply: 10)
627. three-point ligation - (reply: 6)
628. Stability of cut DNA - (reply: 8)
629. problem with transformed plasmid's size - (reply: 10)
630. Can't ligate 2 linear fragments with cohesive ends - (reply: 5)
602. Calculation of MW fusion protein - (reply: 1)
603. Double digestion - (reply: 13)
604. genome walking - (reply: 1)
605. PCR OF HIV genome - (reply: 1)
606. Transformation - (reply: 5)
607. Is time of ligation insert and vector crucial? - (reply: 4)
608. High Throughput transformation - (reply: 5)
609. Retrieve dried vector - (reply: 2)
610. help for no E coli. clone after transforming - (reply: 5)
611. CTAB - (reply: 1)
612. SURE E. coli strain - How make competents cells? (reply: 4)
613. Is this genomic DNA? - or plasmid? (reply: 11)
614. cDNA library insert-vector ratio - (reply: 1)
615. Cloning/Miniprep Blues - High molecular weight bands iand no plasmid n my miniprep (reply: 3)
616. Ligation - (reply: 5)
617. site after blunting - (reply: 1)
618. Cloning w/pCR4 TOPO vector - problems obtaining clones (reply: 1)
619. blunt ending the vector followed by the ligation - (reply: 1)
620. Vector for expressing proteins to be secreted - (reply: 3)
621. Vector for producing Fc fusion proteins - (reply: 1)
622. Multiple fragment ligation - (reply: 7)
623. Kozak sequence between GST and gene of my interest - There is a kozak sequence between GST and my gene. Does it hurt? (reply: 1)
624. Colony PCR - (reply: 11)
625. primer dissolved in wrong buffer (1M Tris HCl) - what to do now?? (reply: 7)
626. HELP: Ligation Problems - (reply: 10)
627. three-point ligation - (reply: 6)
628. Stability of cut DNA - (reply: 8)
629. problem with transformed plasmid's size - (reply: 10)
630. Can't ligate 2 linear fragments with cohesive ends - (reply: 5)