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421. puc19 vector - (reply: 1)
422. false positive in colony screening PCR? - (reply: 8)
423. SAP incubation time - how long do you incubate for?? (reply: 1)
424. Protein expression in E.coli DH5alpha? - (reply: 1)
425. pEGFP N1 vector - (reply: 6)
426. help trouble shooting: PstI/SapI cloning - Is SapI a nasty enzyme? (reply: 6)
427. MiniPrep - (reply: 3)
428. large plasmid extraction? - how? (reply: 5)
429. no plasmid extracted - low copy number - (reply: 2)
430. Help with TOPO TA cloning – too long or too many insert - (reply: 1)
431. Cloning big inserts - (reply: 4)
432. no GFP in cells - (reply: 7)
433. should I cut out signal peptide for expression - (reply: 1)
434. Random Amplification + TA cloning = HATE - (reply: 2)
435. how important a terminator is in cloning? - (reply: 3)
436. Storage of transformation reaction mix (Promega) - CLoning-transformation (reply: 2)
437. strange transformation products in subclone - (reply: 4)
438. Site-Directed Mutagenesis Kit - (reply: 1)
439. basics about expression vectors - (reply: 2)
440. no plasmid after extraction - (reply: 2)
441. help - (reply: 1)
442. BLUNT END LIGATION - DIFFICULTIES ON BLUNT END LIGATIONS (reply: 3)
443. Problem with Cloning- NO colonies at all - (reply: 6)
444. Blunt end cloning - vector and insert preparation (reply: 9)
445. Electroporation with Pseudomonas strains - (reply: 1)
446. P/C/I extraction of plasmid after digestion - (reply: 1)
447. difference between Qiaex and Qiaquick gel extraction kits - (reply: 5)
448. Recommendations for a good site-directed mutagenesis kit - (reply: 3)
449. E. coli stain for cloning that grow up very quick - Any one who could give the info??? (reply: 5)
450. Gel extraction problem ~HELP~ - (reply: 3)
422. false positive in colony screening PCR? - (reply: 8)
423. SAP incubation time - how long do you incubate for?? (reply: 1)
424. Protein expression in E.coli DH5alpha? - (reply: 1)
425. pEGFP N1 vector - (reply: 6)
426. help trouble shooting: PstI/SapI cloning - Is SapI a nasty enzyme? (reply: 6)
427. MiniPrep - (reply: 3)
428. large plasmid extraction? - how? (reply: 5)
429. no plasmid extracted - low copy number - (reply: 2)
430. Help with TOPO TA cloning – too long or too many insert - (reply: 1)
431. Cloning big inserts - (reply: 4)
432. no GFP in cells - (reply: 7)
433. should I cut out signal peptide for expression - (reply: 1)
434. Random Amplification + TA cloning = HATE - (reply: 2)
435. how important a terminator is in cloning? - (reply: 3)
436. Storage of transformation reaction mix (Promega) - CLoning-transformation (reply: 2)
437. strange transformation products in subclone - (reply: 4)
438. Site-Directed Mutagenesis Kit - (reply: 1)
439. basics about expression vectors - (reply: 2)
440. no plasmid after extraction - (reply: 2)
441. help - (reply: 1)
442. BLUNT END LIGATION - DIFFICULTIES ON BLUNT END LIGATIONS (reply: 3)
443. Problem with Cloning- NO colonies at all - (reply: 6)
444. Blunt end cloning - vector and insert preparation (reply: 9)
445. Electroporation with Pseudomonas strains - (reply: 1)
446. P/C/I extraction of plasmid after digestion - (reply: 1)
447. difference between Qiaex and Qiaquick gel extraction kits - (reply: 5)
448. Recommendations for a good site-directed mutagenesis kit - (reply: 3)
449. E. coli stain for cloning that grow up very quick - Any one who could give the info??? (reply: 5)
450. Gel extraction problem ~HELP~ - (reply: 3)