|
961. Insert is double the size than vector - (reply: 6)
962. transformation - random genomic inserts (reply: 3)
963. cDNA problem - (reply: 5)
964. oligo annealing - (reply: 3)
965. Multiple transformation - How many plasmids is it possible to transform at the same time ? (reply: 2)
966. Help with ligation using Topo TA - (reply: 7)
967. Restriction digest - volume of restriction digest (reply: 2)
968. Gene cloning - Gene library (reply: 1)
969. competen cell - (reply: 3)
970. Problem on negative control in Xgal - Question, suggestion (reply: 1)
971. impossible single enzyme cut - (reply: 1)
972. why the insert is always in opposite orientation - (reply: 1)
973. Smear on gel after MiniPrep - Is this RNA? (reply: 10)
974. How to get a plasimd-protein complex? - (reply: 2)
975. what are the HUGE colonies on my negative plates? - (reply: 6)
976. Ethanol precipitation vs Clean up kit? - which will yield more (reply: 16)
977. weird DNA bands in the Minipreps; competent cells contamination? - (reply: 3)
978. which restriction enzyme should I use to digest the vector? - (reply: 3)
979. same vector but diffirent clones produce bands of different size. - (reply: 4)
980. forgot to digest PCR insert before ligation. - what happened? (reply: 7)
981. CIAP treatment for stable transfection ? - (reply: 1)
982. difference between DH5alpha and lamda pair DH5alpha? - (reply: 2)
983. Problem with colony PCR - (reply: 8)
984. 2nd trial of colony-PCR - (reply: 2)
985. update of colony-PCR result --screening for correct recombinants from 4-way liga - (reply: 3)
986. differences between E. coli strains Top10 and DH10B - (reply: 3)
987. Annealing Temp=Extension Temp - (reply: 4)
988. Plasmid DNA problem - PEG precipitation (reply: 3)
989. Conting - (reply: 1)
990. Low yield in maxiprep - (reply: 4)
962. transformation - random genomic inserts (reply: 3)
963. cDNA problem - (reply: 5)
964. oligo annealing - (reply: 3)
965. Multiple transformation - How many plasmids is it possible to transform at the same time ? (reply: 2)
966. Help with ligation using Topo TA - (reply: 7)
967. Restriction digest - volume of restriction digest (reply: 2)
968. Gene cloning - Gene library (reply: 1)
969. competen cell - (reply: 3)
970. Problem on negative control in Xgal - Question, suggestion (reply: 1)
971. impossible single enzyme cut - (reply: 1)
972. why the insert is always in opposite orientation - (reply: 1)
973. Smear on gel after MiniPrep - Is this RNA? (reply: 10)
974. How to get a plasimd-protein complex? - (reply: 2)
975. what are the HUGE colonies on my negative plates? - (reply: 6)
976. Ethanol precipitation vs Clean up kit? - which will yield more (reply: 16)
977. weird DNA bands in the Minipreps; competent cells contamination? - (reply: 3)
978. which restriction enzyme should I use to digest the vector? - (reply: 3)
979. same vector but diffirent clones produce bands of different size. - (reply: 4)
980. forgot to digest PCR insert before ligation. - what happened? (reply: 7)
981. CIAP treatment for stable transfection ? - (reply: 1)
982. difference between DH5alpha and lamda pair DH5alpha? - (reply: 2)
983. Problem with colony PCR - (reply: 8)
984. 2nd trial of colony-PCR - (reply: 2)
985. update of colony-PCR result --screening for correct recombinants from 4-way liga - (reply: 3)
986. differences between E. coli strains Top10 and DH10B - (reply: 3)
987. Annealing Temp=Extension Temp - (reply: 4)
988. Plasmid DNA problem - PEG precipitation (reply: 3)
989. Conting - (reply: 1)
990. Low yield in maxiprep - (reply: 4)