|
1591. Adeno-X Expression System problems - (reply: 3)
1592. purification by gel? - after a first round of PCR (reply: 2)
1593. Experience with NheI, AsiSI, FseI and MluI? - (reply: 4)
1594. Reverse transcriptase PCR - RTPCT (reply: 2)
1595. False positive for colony PCR - (reply: 3)
1596. restriction digest and DNA degradation - (reply: 3)
1597. ligating small oligos - how to ligate small oligos into vector (reply: 2)
1598. what's wrong with my digestion - (reply: 2)
1599. ligation question - (reply: 6)
1600. Ligation problem - (reply: 1)
1601. linearisation of plasmid - pBS never gets cut :-( (reply: 14)
1602. Mystery band after vector digestion - (reply: 3)
1603. putting BAC plasmid back into E. coli - (reply: 7)
1604. digestion with two REs requiring different buffer - (reply: 10)
1605. What way would you suggest to clean PCR products? - (reply: 4)
1606. Can I make four substitutions (mutagenesis) using PCR at a time? - help me out, pls :) (reply: 7)
1607. ligation of short oligos - (reply: 3)
1608. How to make a point mutation in a wild type plasmid. - (reply: 2)
1609. blunt ended cloning problem - (reply: 7)
1610. Sequential digestion - Sequential digestion using two RE with different incubation temperatures and dif (reply: 11)
1611. Double-digesting my vector - should I see a 76bp band? (reply: 6)
1612. How to purify and clone a 88 bp fragment? - (reply: 4)
1613. question on carrier salmon sperm DNA - (reply: 5)
1614. I lost my insert! - (reply: 22)
1615. cloning of PCR product - (reply: 1)
1616. e-value in BLASTn search - (reply: 2)
1617. Colony selection - (reply: 7)
1618. transformation colony - how to get rid of background colonies? (reply: 2)
1619. problems with digesting insert from vector - (reply: 4)
1620. cDNA cloning question - (reply: 7)
1592. purification by gel? - after a first round of PCR (reply: 2)
1593. Experience with NheI, AsiSI, FseI and MluI? - (reply: 4)
1594. Reverse transcriptase PCR - RTPCT (reply: 2)
1595. False positive for colony PCR - (reply: 3)
1596. restriction digest and DNA degradation - (reply: 3)
1597. ligating small oligos - how to ligate small oligos into vector (reply: 2)
1598. what's wrong with my digestion - (reply: 2)
1599. ligation question - (reply: 6)
1600. Ligation problem - (reply: 1)
1601. linearisation of plasmid - pBS never gets cut :-( (reply: 14)
1602. Mystery band after vector digestion - (reply: 3)
1603. putting BAC plasmid back into E. coli - (reply: 7)
1604. digestion with two REs requiring different buffer - (reply: 10)
1605. What way would you suggest to clean PCR products? - (reply: 4)
1606. Can I make four substitutions (mutagenesis) using PCR at a time? - help me out, pls :) (reply: 7)
1607. ligation of short oligos - (reply: 3)
1608. How to make a point mutation in a wild type plasmid. - (reply: 2)
1609. blunt ended cloning problem - (reply: 7)
1610. Sequential digestion - Sequential digestion using two RE with different incubation temperatures and dif (reply: 11)
1611. Double-digesting my vector - should I see a 76bp band? (reply: 6)
1612. How to purify and clone a 88 bp fragment? - (reply: 4)
1613. question on carrier salmon sperm DNA - (reply: 5)
1614. I lost my insert! - (reply: 22)
1615. cloning of PCR product - (reply: 1)
1616. e-value in BLASTn search - (reply: 2)
1617. Colony selection - (reply: 7)
1618. transformation colony - how to get rid of background colonies? (reply: 2)
1619. problems with digesting insert from vector - (reply: 4)
1620. cDNA cloning question - (reply: 7)