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781. Heating the vector and insert before ligation - new idea for reducing background colonies (reply: 7)
782. two fragment ligation - (reply: 3)
783. PCR - (reply: 5)
784. Ligation of half blunt PCR fragments - (reply: 4)
785. making competent SURE cells - (reply: 2)
786. cloning primers, TM, and specificity - (reply: 4)
787. Digestion, Ligation or transformation problem? - (reply: 8)
788. Directional cloning - (reply: 4)
789. Cloning problems into a big (19 kb) vector - (reply: 10)
790. Promoter experiments - What's next? (reply: 2)
791. Stability of a transgene and its expression - (reply: 3)
792. non radioactive for southern blot - (reply: 3)
793. bacterial transformation - (reply: 4)
794. Promega :Mutagenesis Systems - (reply: 5)
795. Strategies for gene cloning and expression - Discuss various methods for it (reply: 8)
796. Help with fusion PCR to piece together fragments - (reply: 5)
797. Modification of Vector - (reply: 1)
798. Weird transformation products - (reply: 5)
799. pgh19 Xenopus expression vector - Need sequence (reply: 3)
800. Making more empty vector - (reply: 7)
801. Making Competent Cells - DH5A vs JM109 or anyother (reply: 5)
802. two additional amino acids in gene - (reply: 4)
803. After successful Transfection protein not detectable - (reply: 3)
804. PCR Amplification from clones - (reply: 7)
805. Annealing and cloning of four single stranded oligos - (reply: 1)
806. Excision of foreign genes by E.coli - suspicion of attack (reply: 5)
807. how to purify plasmid and genomic dna - (reply: 3)
808. does size matter - how many genes in 1 vector (reply: 10)
809. Religated vector despite a "no insert control" - help! - (reply: 2)
810. insert to vector ratio - (reply: 3)
782. two fragment ligation - (reply: 3)
783. PCR - (reply: 5)
784. Ligation of half blunt PCR fragments - (reply: 4)
785. making competent SURE cells - (reply: 2)
786. cloning primers, TM, and specificity - (reply: 4)
787. Digestion, Ligation or transformation problem? - (reply: 8)
788. Directional cloning - (reply: 4)
789. Cloning problems into a big (19 kb) vector - (reply: 10)
790. Promoter experiments - What's next? (reply: 2)
791. Stability of a transgene and its expression - (reply: 3)
792. non radioactive for southern blot - (reply: 3)
793. bacterial transformation - (reply: 4)
794. Promega :Mutagenesis Systems - (reply: 5)
795. Strategies for gene cloning and expression - Discuss various methods for it (reply: 8)
796. Help with fusion PCR to piece together fragments - (reply: 5)
797. Modification of Vector - (reply: 1)
798. Weird transformation products - (reply: 5)
799. pgh19 Xenopus expression vector - Need sequence (reply: 3)
800. Making more empty vector - (reply: 7)
801. Making Competent Cells - DH5A vs JM109 or anyother (reply: 5)
802. two additional amino acids in gene - (reply: 4)
803. After successful Transfection protein not detectable - (reply: 3)
804. PCR Amplification from clones - (reply: 7)
805. Annealing and cloning of four single stranded oligos - (reply: 1)
806. Excision of foreign genes by E.coli - suspicion of attack (reply: 5)
807. how to purify plasmid and genomic dna - (reply: 3)
808. does size matter - how many genes in 1 vector (reply: 10)
809. Religated vector despite a "no insert control" - help! - (reply: 2)
810. insert to vector ratio - (reply: 3)