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991. genomic insert ligation - (reply: 3)
992. Restriction site clonning failed - (reply: 9)
993. one question on the 4-way ligation - (reply: 10)
994. a large insert ligation - (reply: 2)
995. Options for protein expression? - (reply: 3)
996. checking the direction of the insert - (reply: 5)
997. Primer design with restriction sites - (reply: 3)
998. ligation - (reply: 3)
999. Knock out in Operon systems - Advice (reply: 1)
1000. translation of mRNA into protein - (reply: 1)
1001. Cloning and Expression vectors - Which is the best Expression vector to use? (reply: 3)
1002. Fuzzy bands on agarose gel after ligation - (reply: 7)
1003. ligation ratio insert-vector - (reply: 6)
1004. Struggle for constructs with pLENTI - (reply: 3)
1005. substitution for PMSF? - (reply: 6)
1006. ligation: only false positives. Advice needed - (reply: 2)
1007. can I take some of ligation mix and digest? - (reply: 4)
1008. problems with miniprep result - (reply: 1)
1009. DIG Southern: can Ethidium Bromide be a problem? - (reply: 3)
1010. cut-delete-insert once more - just cloning? - (reply: 3)
1011. inclusion of translation start site when cloning promoter insert - (reply: 3)
1012. digest, blunt end, religate, digest.... - so many steps, maybe can skip purification... (reply: 2)
1013. cloning experimental design to ligate three PCR products into one plasmid - (reply: 4)
1014. possible contamination of TA cloning vector? - (reply: 4)
1015. Competant cells & incompetant scientist - No colonies formed (reply: 8)
1016. Explanation of DNA Precipitation - (reply: 4)
1017. How big can be the insert? - (reply: 1)
1018. electroporation cuvettes-single use only? - (reply: 9)
1019. After double digest my plasmid is 2Kbp shorter! - (reply: 3)
1020. Map of MIGR1 retroviral vector needed - (reply: 1)
992. Restriction site clonning failed - (reply: 9)
993. one question on the 4-way ligation - (reply: 10)
994. a large insert ligation - (reply: 2)
995. Options for protein expression? - (reply: 3)
996. checking the direction of the insert - (reply: 5)
997. Primer design with restriction sites - (reply: 3)
998. ligation - (reply: 3)
999. Knock out in Operon systems - Advice (reply: 1)
1000. translation of mRNA into protein - (reply: 1)
1001. Cloning and Expression vectors - Which is the best Expression vector to use? (reply: 3)
1002. Fuzzy bands on agarose gel after ligation - (reply: 7)
1003. ligation ratio insert-vector - (reply: 6)
1004. Struggle for constructs with pLENTI - (reply: 3)
1005. substitution for PMSF? - (reply: 6)
1006. ligation: only false positives. Advice needed - (reply: 2)
1007. can I take some of ligation mix and digest? - (reply: 4)
1008. problems with miniprep result - (reply: 1)
1009. DIG Southern: can Ethidium Bromide be a problem? - (reply: 3)
1010. cut-delete-insert once more - just cloning? - (reply: 3)
1011. inclusion of translation start site when cloning promoter insert - (reply: 3)
1012. digest, blunt end, religate, digest.... - so many steps, maybe can skip purification... (reply: 2)
1013. cloning experimental design to ligate three PCR products into one plasmid - (reply: 4)
1014. possible contamination of TA cloning vector? - (reply: 4)
1015. Competant cells & incompetant scientist - No colonies formed (reply: 8)
1016. Explanation of DNA Precipitation - (reply: 4)
1017. How big can be the insert? - (reply: 1)
1018. electroporation cuvettes-single use only? - (reply: 9)
1019. After double digest my plasmid is 2Kbp shorter! - (reply: 3)
1020. Map of MIGR1 retroviral vector needed - (reply: 1)