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1081. How to determine PCR Annealing temperature for primers with different Tm - (reply: 7)
1082. digesting out restriction site - (reply: 6)
1083. cDNA sequences too similar to be real? - (reply: 2)
1084. Molecular cloning of SalI/PacI digested product in modified pBluescript backbone - (reply: 4)
1085. Minimum sample required for visualization on gel - How to load 1ul of DNA on gel (reply: 3)
1086. in vitro transcription with T7 promoter - (reply: 3)
1087. preparation of electrocompetant cells. - what about the density? (reply: 3)
1088. Cloning WITHOUT Topoisomerase! - Does any one know of an expression vector that does not depend on Topoisomerase (reply: 13)
1089. cloning problem with pcr and pegfp vector - (reply: 9)
1090. problems with cloning into a pET15b vector - (reply: 2)
1091. TOPO CLONING - (reply: 5)
1092. Identify insert by restriction digestion - (reply: 6)
1093. how to get cosmid DNA back to E.coli? - (reply: 3)
1094. VECTOR LIGATION WITH One RE - (reply: 4)
1095. dimer in vectors - (reply: 2)
1096. LacZ fusion with pRS415 - Protocol (reply: 2)
1097. Afl II linearization & ligation - (reply: 3)
1098. LOOKING for vector map and sequence of pSVaZ11 - (reply: 1)
1099. false positives when cloning microsatellite fragments - please help! - (reply: 3)
1100. PCR: same premix, same everything, different results... - (reply: 21)
1101. colony PCR-sometimes works, sometimes doesn't :( - (reply: 3)
1102. Replacing of GFP cloned inside a gene with RFP - (reply: 2)
1103. Expression of fusion protein in mammalian cells - (reply: 2)
1104. Cloning into expression vector - Why doesn't it work anymore? (reply: 7)
1105. PCR shows insert, but digestion of plasmid failed to cut out insert - (reply: 2)
1106. A new plasmid appears on my gel in addition to my construct when I increase the - (reply: 4)
1107. pGEM-T Restriction Enzymes won't cut out PCR clone - (reply: 3)
1108. is there any way to "hide" one restriction site of two in order to dig - (reply: 3)
1109. Should I purify digested vector before ligation? - (reply: 7)
1110. Ligation and cloning failure - (reply: 3)
1082. digesting out restriction site - (reply: 6)
1083. cDNA sequences too similar to be real? - (reply: 2)
1084. Molecular cloning of SalI/PacI digested product in modified pBluescript backbone - (reply: 4)
1085. Minimum sample required for visualization on gel - How to load 1ul of DNA on gel (reply: 3)
1086. in vitro transcription with T7 promoter - (reply: 3)
1087. preparation of electrocompetant cells. - what about the density? (reply: 3)
1088. Cloning WITHOUT Topoisomerase! - Does any one know of an expression vector that does not depend on Topoisomerase (reply: 13)
1089. cloning problem with pcr and pegfp vector - (reply: 9)
1090. problems with cloning into a pET15b vector - (reply: 2)
1091. TOPO CLONING - (reply: 5)
1092. Identify insert by restriction digestion - (reply: 6)
1093. how to get cosmid DNA back to E.coli? - (reply: 3)
1094. VECTOR LIGATION WITH One RE - (reply: 4)
1095. dimer in vectors - (reply: 2)
1096. LacZ fusion with pRS415 - Protocol (reply: 2)
1097. Afl II linearization & ligation - (reply: 3)
1098. LOOKING for vector map and sequence of pSVaZ11 - (reply: 1)
1099. false positives when cloning microsatellite fragments - please help! - (reply: 3)
1100. PCR: same premix, same everything, different results... - (reply: 21)
1101. colony PCR-sometimes works, sometimes doesn't :( - (reply: 3)
1102. Replacing of GFP cloned inside a gene with RFP - (reply: 2)
1103. Expression of fusion protein in mammalian cells - (reply: 2)
1104. Cloning into expression vector - Why doesn't it work anymore? (reply: 7)
1105. PCR shows insert, but digestion of plasmid failed to cut out insert - (reply: 2)
1106. A new plasmid appears on my gel in addition to my construct when I increase the - (reply: 4)
1107. pGEM-T Restriction Enzymes won't cut out PCR clone - (reply: 3)
1108. is there any way to "hide" one restriction site of two in order to dig - (reply: 3)
1109. Should I purify digested vector before ligation? - (reply: 7)
1110. Ligation and cloning failure - (reply: 3)