|
1681. Who knows and uses the Plasmid pGag-PolGpt? - Poor plasmid yield with Maxi-Prep.... (reply: 1)
1682. Double digestion of PCR product and ligation - (reply: 5)
1683. HELP! eliminating A overhangs - (reply: 1)
1684. adding A overhangs - how much dATP? (reply: 4)
1685. help needed for large construct cloning - (reply: 6)
1686. cloning problem - sticky end cloning with PCR insert - (reply: 3)
1687. How do I make more competent cells? - by growing up and freezing glycerol stock? (reply: 39)
1688. subcloning into a single NotI site - (reply: 11)
1689. PCR colony screening - (reply: 9)
1690. How to construct T vector - (reply: 8)
1691. poor ligation of plasmid/oligo construct - (reply: 8)
1692. Dephosphorylation of vector before ligation - Is it required? (reply: 38)
1693. Alternative to pIRES cloning - Looking for methods other than pIRES (reply: 2)
1694. need help in molecular cloning - molecular biology (reply: 6)
1695. How to ligate ds-oligos into a vector? - (reply: 48)
1696. cloning from PCR - (reply: 25)
1697. Double digestion and ligation problem - (reply: 15)
1698. blunt-cloning - all are vector self-ligation products? (reply: 33)
1699. Plasmid DNA extraction - Extracted plasmid cannot be seen in agarose gel (reply: 9)
1700. plasmid can't be digested by enzyme - (reply: 13)
1682. Double digestion of PCR product and ligation - (reply: 5)
1683. HELP! eliminating A overhangs - (reply: 1)
1684. adding A overhangs - how much dATP? (reply: 4)
1685. help needed for large construct cloning - (reply: 6)
1686. cloning problem - sticky end cloning with PCR insert - (reply: 3)
1687. How do I make more competent cells? - by growing up and freezing glycerol stock? (reply: 39)
1688. subcloning into a single NotI site - (reply: 11)
1689. PCR colony screening - (reply: 9)
1690. How to construct T vector - (reply: 8)
1691. poor ligation of plasmid/oligo construct - (reply: 8)
1692. Dephosphorylation of vector before ligation - Is it required? (reply: 38)
1693. Alternative to pIRES cloning - Looking for methods other than pIRES (reply: 2)
1694. need help in molecular cloning - molecular biology (reply: 6)
1695. How to ligate ds-oligos into a vector? - (reply: 48)
1696. cloning from PCR - (reply: 25)
1697. Double digestion and ligation problem - (reply: 15)
1698. blunt-cloning - all are vector self-ligation products? (reply: 33)
1699. Plasmid DNA extraction - Extracted plasmid cannot be seen in agarose gel (reply: 9)
1700. plasmid can't be digested by enzyme - (reply: 13)