|
1381. PCR of long fragment(approx 8 kb) - (reply: 3)
1382. Is re-sequencing needed for insert ligated into a second vector? - (reply: 1)
1383. question on TOPO TA problems - no positive colonies - (reply: 4)
1384. Protocol for insert orientation - (reply: 1)
1385. basic question - (reply: 1)
1386. Host cells for pBELOBAC11 - (reply: 4)
1387. Is A-tailing of restriction fragment possible? - (reply: 2)
1388. why SOC medium is better? - (reply: 1)
1389. how to confirm ligation is successful - (reply: 3)
1390. colonies - (reply: 4)
1391. lousy agarose gel - (reply: 2)
1392. Topo tools - need ur help (reply: 2)
1393. TA cloning-Insert size too big? - (reply: 2)
1394. cloning the promoter from genomic dna - (reply: 9)
1395. single clone isolate showed multiple bands in DGGE - (reply: 1)
1396. Intron detection - (reply: 1)
1397. How to extract a 20kb vector after double digestion - (reply: 4)
1398. What'sa phagemid? - phagemid cloning (reply: 3)
1399. Cloning Problem - (reply: 5)
1400. Cloning gene behind GFP - Vector? (reply: 2)
1401. Gateway cloning - help (reply: 13)
1402. purification of DNA extracted from agarose gel - does ethidium bromide remains / interefrence with ligation (reply: 1)
1403. pRSETA - (reply: 1)
1404. Cloning and Charecterization of a Novel Receptor - (reply: 1)
1405. inserts and vector ratio in liagtion Rxns - (reply: 6)
1406. Transfection into a cell line which already express your protein - (reply: 3)
1407. pUWL501 - Restriction map (reply: 3)
1408. plasmid sterilization - (reply: 1)
1409. Gel Extraction problems - using Invitrogen Purelink (reply: 1)
1410. Genetic Manipulation - (reply: 2)
1382. Is re-sequencing needed for insert ligated into a second vector? - (reply: 1)
1383. question on TOPO TA problems - no positive colonies - (reply: 4)
1384. Protocol for insert orientation - (reply: 1)
1385. basic question - (reply: 1)
1386. Host cells for pBELOBAC11 - (reply: 4)
1387. Is A-tailing of restriction fragment possible? - (reply: 2)
1388. why SOC medium is better? - (reply: 1)
1389. how to confirm ligation is successful - (reply: 3)
1390. colonies - (reply: 4)
1391. lousy agarose gel - (reply: 2)
1392. Topo tools - need ur help (reply: 2)
1393. TA cloning-Insert size too big? - (reply: 2)
1394. cloning the promoter from genomic dna - (reply: 9)
1395. single clone isolate showed multiple bands in DGGE - (reply: 1)
1396. Intron detection - (reply: 1)
1397. How to extract a 20kb vector after double digestion - (reply: 4)
1398. What'sa phagemid? - phagemid cloning (reply: 3)
1399. Cloning Problem - (reply: 5)
1400. Cloning gene behind GFP - Vector? (reply: 2)
1401. Gateway cloning - help (reply: 13)
1402. purification of DNA extracted from agarose gel - does ethidium bromide remains / interefrence with ligation (reply: 1)
1403. pRSETA - (reply: 1)
1404. Cloning and Charecterization of a Novel Receptor - (reply: 1)
1405. inserts and vector ratio in liagtion Rxns - (reply: 6)
1406. Transfection into a cell line which already express your protein - (reply: 3)
1407. pUWL501 - Restriction map (reply: 3)
1408. plasmid sterilization - (reply: 1)
1409. Gel Extraction problems - using Invitrogen Purelink (reply: 1)
1410. Genetic Manipulation - (reply: 2)