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1471. question for all you cloning gurus... - (reply: 8)
1472. [32] phosphorylation hints needed! - (reply: 1)
1473. Cloning a 2kb fragment! - (reply: 2)
1474. do primers and dNTPs alter digestion efficiency? - (reply: 4)
1475. isolation of DNA from a plasmid for site-directed mutagenesis? - do researchers actually do this? (reply: 6)
1476. is it my recombinant plasmid or contamination? - only two colonies on the plate.... (reply: 5)
1477. Do I need CIP before my ligation - (reply: 4)
1478. cloning 11 kb fragment - (reply: 2)
1479. transformation of a big plasmid in E. coli - something wrong is happening in my transformation (reply: 6)
1480. Sequencing primer dilution - (reply: 2)
1481. magic? - got more product after PCR purification... (reply: 3)
1482. plasmid map software? - (reply: 7)
1483. Salt problem in cosmid DNA extraction - how to remove excess salts (reply: 2)
1484. Water or TSB to dilute an over night culture!? - (reply: 6)
1485. cloning vector - cloning vector (reply: 1)
1486. Miniprep problem - (reply: 9)
1487. which vector will be best for cloning and then expression - vector (reply: 1)
1488. How can I find the sequence after and before my interested ORF? - finding sesequence after and before an ORF? (reply: 4)
1489. Plz help me troubleshoot subcloning! - (reply: 6)
1490. Blunt ended ligation - (reply: 7)
1491. Maxi prep problems - (reply: 5)
1492. what is the best pair of sequencing primer for puc 18 - (reply: 1)
1493. pCI sequencing primer - (reply: 1)
1494. pBR322 relaxed plasmid....what type of media? - (reply: 3)
1495. pQE30 sequence needed - or restriction map (reply: 3)
1496. Anyone has the experience to order plasmid from "Addgene"? - (reply: 2)
1497. ligation problems - (reply: 8)
1498. RNA extraction from blood - (reply: 9)
1499. how to confirm if ligation is sucessful? - (reply: 4)
1500. To inactivate or not to inactivate ligase before transformation? - (reply: 5)
1472. [32] phosphorylation hints needed! - (reply: 1)
1473. Cloning a 2kb fragment! - (reply: 2)
1474. do primers and dNTPs alter digestion efficiency? - (reply: 4)
1475. isolation of DNA from a plasmid for site-directed mutagenesis? - do researchers actually do this? (reply: 6)
1476. is it my recombinant plasmid or contamination? - only two colonies on the plate.... (reply: 5)
1477. Do I need CIP before my ligation - (reply: 4)
1478. cloning 11 kb fragment - (reply: 2)
1479. transformation of a big plasmid in E. coli - something wrong is happening in my transformation (reply: 6)
1480. Sequencing primer dilution - (reply: 2)
1481. magic? - got more product after PCR purification... (reply: 3)
1482. plasmid map software? - (reply: 7)
1483. Salt problem in cosmid DNA extraction - how to remove excess salts (reply: 2)
1484. Water or TSB to dilute an over night culture!? - (reply: 6)
1485. cloning vector - cloning vector (reply: 1)
1486. Miniprep problem - (reply: 9)
1487. which vector will be best for cloning and then expression - vector (reply: 1)
1488. How can I find the sequence after and before my interested ORF? - finding sesequence after and before an ORF? (reply: 4)
1489. Plz help me troubleshoot subcloning! - (reply: 6)
1490. Blunt ended ligation - (reply: 7)
1491. Maxi prep problems - (reply: 5)
1492. what is the best pair of sequencing primer for puc 18 - (reply: 1)
1493. pCI sequencing primer - (reply: 1)
1494. pBR322 relaxed plasmid....what type of media? - (reply: 3)
1495. pQE30 sequence needed - or restriction map (reply: 3)
1496. Anyone has the experience to order plasmid from "Addgene"? - (reply: 2)
1497. ligation problems - (reply: 8)
1498. RNA extraction from blood - (reply: 9)
1499. how to confirm if ligation is sucessful? - (reply: 4)
1500. To inactivate or not to inactivate ligase before transformation? - (reply: 5)