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Molecular-Cloning
1531.
Ligation/Transformation problem -
(reply: 4)
1532.
pcDNA 3.1/pTarget and stable cell line -
(reply: 10)
1533.
vector with tag recommendation -
(reply: 10)
1534.
Vector databases and resources -
(reply: 6)
1535.
Topo-Cloning over 3 kb -
(reply: 5)
1536.
why the inserted fragment can not be cutted ..... - the inserted fragment can not cutted by the enzymes used
(reply: 1)
1537.
need help,why colony pcr of my blue has product what i need -
(reply: 2)
1538.
Double Digestion and Single Digestion same result? - Confusion about Plasmid and Insert alignment
(reply: 4)
1539.
Types of DNA polymerases that you use. -
(reply: 3)
1540.
so lost! stupid quenstion about primers.... - designing the primers
(reply: 2)
1541.
Question about Nox gene -
(reply: 1)
1542.
designing the sequencing primer -
(reply: 2)
1543.
Mutagenic PCR unsuccessful :( - pls help....
(reply: 7)
1544.
Me again...on type of polymerase and REs I should use... -
(reply: 6)
1545.
design of PRC primers of full ORF -
(reply: 3)
1546.
Cloning - how to avoid religation and no insert? -
(reply: 13)
1547.
RT-PCR with an old DNaseI? -
(reply: 3)
1548.
deletion during cloning -
(reply: 3)
1549.
Transformation -
(reply: 4)
1550.
Strange question regarding DNA in gel-slice... -
(reply: 12)
1551.
Vector insertion for beginners -
(reply: 4)
1552.
Ligation help needed! - vector religation?
(reply: 3)
1553.
purification of long single stranded DNA -
(reply: 4)
1554.
gene clone -
(reply: 1)
1555.
No insert for cloning PCR product into expression vector -
(reply: 5)
1556.
Which site of NDV NP gene is cut by BamHI and XhoI? -
(reply: 2)
1557.
ordering a cDNA clone - found on the NCBI database
(reply: 3)
1558.
how to improve ligation efficiency -
(reply: 7)
1559.
really dumb question that's making me batty -
(reply: 6)
1560.
How does IPTG work ? -
(reply: 1)
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