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1531. Ligation/Transformation problem - (reply: 4)
1532. pcDNA 3.1/pTarget and stable cell line - (reply: 10)
1533. vector with tag recommendation - (reply: 10)
1534. Vector databases and resources - (reply: 6)
1535. Topo-Cloning over 3 kb - (reply: 5)
1536. why the inserted fragment can not be cutted ..... - the inserted fragment can not cutted by the enzymes used (reply: 1)
1537. need help,why colony pcr of my blue has product what i need - (reply: 2)
1538. Double Digestion and Single Digestion same result? - Confusion about Plasmid and Insert alignment (reply: 4)
1539. Types of DNA polymerases that you use. - (reply: 3)
1540. so lost! stupid quenstion about primers.... - designing the primers (reply: 2)
1541. Question about Nox gene - (reply: 1)
1542. designing the sequencing primer - (reply: 2)
1543. Mutagenic PCR unsuccessful :( - pls help.... (reply: 7)
1544. Me again...on type of polymerase and REs I should use... - (reply: 6)
1545. design of PRC primers of full ORF - (reply: 3)
1546. Cloning - how to avoid religation and no insert? - (reply: 13)
1547. RT-PCR with an old DNaseI? - (reply: 3)
1548. deletion during cloning - (reply: 3)
1549. Transformation - (reply: 4)
1550. Strange question regarding DNA in gel-slice... - (reply: 12)
1551. Vector insertion for beginners - (reply: 4)
1552. Ligation help needed! - vector religation? (reply: 3)
1553. purification of long single stranded DNA - (reply: 4)
1554. gene clone - (reply: 1)
1555. No insert for cloning PCR product into expression vector - (reply: 5)
1556. Which site of NDV NP gene is cut by BamHI and XhoI? - (reply: 2)
1557. ordering a cDNA clone - found on the NCBI database (reply: 3)
1558. how to improve ligation efficiency - (reply: 7)
1559. really dumb question that's making me batty - (reply: 6)
1560. How does IPTG work ? - (reply: 1)