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151. Why was one part of insert missing? - (reply: 11)
152. Transformation - thanks for help (reply: 6)
153. Will insert increase empty vecotr recircularization? - (reply: 4)
154. gel purifications of DNA fragm > 10Kb - (reply: 9)
155. recombinant human antibody production problem - (reply: 2)
156. adding amino acids - (reply: 3)
157. URGENT: Hepes Buffered saline reagent - (reply: 1)
158. qPCR - (reply: 1)
159. Transformants pictures in gel - (reply: 2)
160. Cloning: blunt and sticky - (reply: 10)
161. purifying small DNA fragmets - (reply: 2)
162. pGEX-2T - (reply: 1)
163. DNA sequencing downstream work - (reply: 3)
164. how to extract DNA from adenovirus for template of PCR - (reply: 2)
165. Colony PCR with Phusion - (reply: 7)
166. Residues don't match... why? - in human and mouse proteins (reply: 2)
167. XhoI! really a bad site? - (reply: 6)
168. Blunting by Klenow - Excess dNTPs (reply: 2)
169. Multigene cloning - Looking for a cheap solution - (reply: 4)
170. Domain-Exchange via Blunt End Cloning of 2 PCR-Products - (reply: 4)
171. Rearrangement in plasmid? - (reply: 2)
172. expression by hsp60 in E.coli - expressions (reply: 1)
173. Store restrict Enzyme in working system - (reply: 5)
174. restriction digestion of plasmid - (reply: 8)
175. Ligation problem - (reply: 7)
176. Two restriction sites are close each other - Can I use them?? (reply: 3)
177. How to improve DNase efficiency after in vitro transcription? - (reply: 3)
178. Transformation troubleshoot - (reply: 7)
179. Subcloning problem - (reply: 9)
180. Purified cDNA Clonning - (reply: 3)
152. Transformation - thanks for help (reply: 6)
153. Will insert increase empty vecotr recircularization? - (reply: 4)
154. gel purifications of DNA fragm > 10Kb - (reply: 9)
155. recombinant human antibody production problem - (reply: 2)
156. adding amino acids - (reply: 3)
157. URGENT: Hepes Buffered saline reagent - (reply: 1)
158. qPCR - (reply: 1)
159. Transformants pictures in gel - (reply: 2)
160. Cloning: blunt and sticky - (reply: 10)
161. purifying small DNA fragmets - (reply: 2)
162. pGEX-2T - (reply: 1)
163. DNA sequencing downstream work - (reply: 3)
164. how to extract DNA from adenovirus for template of PCR - (reply: 2)
165. Colony PCR with Phusion - (reply: 7)
166. Residues don't match... why? - in human and mouse proteins (reply: 2)
167. XhoI! really a bad site? - (reply: 6)
168. Blunting by Klenow - Excess dNTPs (reply: 2)
169. Multigene cloning - Looking for a cheap solution - (reply: 4)
170. Domain-Exchange via Blunt End Cloning of 2 PCR-Products - (reply: 4)
171. Rearrangement in plasmid? - (reply: 2)
172. expression by hsp60 in E.coli - expressions (reply: 1)
173. Store restrict Enzyme in working system - (reply: 5)
174. restriction digestion of plasmid - (reply: 8)
175. Ligation problem - (reply: 7)
176. Two restriction sites are close each other - Can I use them?? (reply: 3)
177. How to improve DNase efficiency after in vitro transcription? - (reply: 3)
178. Transformation troubleshoot - (reply: 7)
179. Subcloning problem - (reply: 9)
180. Purified cDNA Clonning - (reply: 3)