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1051. Possible to clone when you have only 20ng PCR product? - (reply: 10)
1052. can colony-PCR screen for big inserts? - (reply: 3)
1053. TOPO cloning always gets wrong orientation - (reply: 3)
1054. Multiway Ligations; sharing the art - Three way ligation and above (reply: 3)
1055. EGFP and GFP - how different are they? (reply: 8)
1056. Is dephosphate needed? - (reply: 6)
1057. advice on construct - possible problem with vector (reply: 7)
1058. Restriction digestion by SpeI and SphI - (reply: 2)
1059. any good method for rapid screening for recombinant plasmids? - (reply: 5)
1060. High transformation background in negative control - (reply: 8)
1061. gateway system - how it works? (reply: 5)
1062. Amplifying a 6.5 kb cDNA - (reply: 3)
1063. E-coli strain and plasmid incompatibility...? - is there something like that? (reply: 14)
1064. pBluescript cloning of PCR products - (reply: 1)
1065. Ligating blunt adapter to PCR product - (reply: 7)
1066. Primer Extension Assay versus RACE-PCR - Protocol (reply: 1)
1067. please tell me more about primers with restriction site! - (reply: 4)
1068. Cloning Construction of 9000bp vector with 900bp - (reply: 14)
1069. isolating large plasmids - is it possible (reply: 12)
1070. Reason for white colonies on outer rim of plates - (reply: 9)
1071. DNA extraction with phenol alone or phenol-chloroform - (reply: 2)
1072. SEAP Assay for promoter activity - (reply: 2)
1073. sequencing is alrite but still...... - (reply: 7)
1074. From yeast to mamall cells - principles and methods online? (reply: 7)
1075. Weird sequencing result of my constructs - (reply: 4)
1076. E. coli strains ER2566 - characteristic or manual? (reply: 2)
1077. Protein expression troubleshooting - problem in expression of my protein (reply: 3)
1078. Few colonies with cloning of PCR product after digestion - (reply: 7)
1079. ligase inhibitors - (reply: 5)
1080. subclone a double-cut insert - (reply: 4)
1052. can colony-PCR screen for big inserts? - (reply: 3)
1053. TOPO cloning always gets wrong orientation - (reply: 3)
1054. Multiway Ligations; sharing the art - Three way ligation and above (reply: 3)
1055. EGFP and GFP - how different are they? (reply: 8)
1056. Is dephosphate needed? - (reply: 6)
1057. advice on construct - possible problem with vector (reply: 7)
1058. Restriction digestion by SpeI and SphI - (reply: 2)
1059. any good method for rapid screening for recombinant plasmids? - (reply: 5)
1060. High transformation background in negative control - (reply: 8)
1061. gateway system - how it works? (reply: 5)
1062. Amplifying a 6.5 kb cDNA - (reply: 3)
1063. E-coli strain and plasmid incompatibility...? - is there something like that? (reply: 14)
1064. pBluescript cloning of PCR products - (reply: 1)
1065. Ligating blunt adapter to PCR product - (reply: 7)
1066. Primer Extension Assay versus RACE-PCR - Protocol (reply: 1)
1067. please tell me more about primers with restriction site! - (reply: 4)
1068. Cloning Construction of 9000bp vector with 900bp - (reply: 14)
1069. isolating large plasmids - is it possible (reply: 12)
1070. Reason for white colonies on outer rim of plates - (reply: 9)
1071. DNA extraction with phenol alone or phenol-chloroform - (reply: 2)
1072. SEAP Assay for promoter activity - (reply: 2)
1073. sequencing is alrite but still...... - (reply: 7)
1074. From yeast to mamall cells - principles and methods online? (reply: 7)
1075. Weird sequencing result of my constructs - (reply: 4)
1076. E. coli strains ER2566 - characteristic or manual? (reply: 2)
1077. Protein expression troubleshooting - problem in expression of my protein (reply: 3)
1078. Few colonies with cloning of PCR product after digestion - (reply: 7)
1079. ligase inhibitors - (reply: 5)
1080. subclone a double-cut insert - (reply: 4)