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1021. DIG Southern: can Ethidium Bromide be a problem? - (reply: 3)
1022. cut-delete-insert once more - just cloning? - (reply: 3)
1023. inclusion of translation start site when cloning promoter insert - (reply: 3)
1024. digest, blunt end, religate, digest.... - so many steps, maybe can skip purification... (reply: 2)
1025. cloning experimental design to ligate three PCR products into one plasmid - (reply: 4)
1026. possible contamination of TA cloning vector? - (reply: 4)
1027. Competant cells & incompetant scientist - No colonies formed (reply: 8)
1028. Explanation of DNA Precipitation - (reply: 4)
1029. How big can be the insert? - (reply: 1)
1030. electroporation cuvettes-single use only? - (reply: 9)
1031. After double digest my plasmid is 2Kbp shorter! - (reply: 3)
1032. Map of MIGR1 retroviral vector needed - (reply: 1)
1033. why competent cells take up only 1 type of plasmids? - (reply: 7)
1034. pXT7 vector map - (reply: 3)
1035. Ecoli genomic recombination causing cloning problems - after ligation vector size and sequence change (reply: 5)
1036. cloning of Poly A tail - (reply: 2)
1037. one blunt and one sticky ligation...something wrong - HELP!!! (reply: 2)
1038. help in cloning the promoter region of a gene - (reply: 3)
1039. wrong sequencing result?! - (reply: 13)
1040. Could anyone tell me where I can buy XL10-GOLD strain from? - (reply: 6)
1041. miniprep: no precipitate after PEG treatment. - (reply: 4)
1042. NAME OF SOB MEDIUM - Does anybody know where to find what "SOB" or "SOC" stands f (reply: 1)
1043. Cutting out of a T vector - Cutting sites in primers vs sites in vector (reply: 3)
1044. Ligation problem, might be bc/ it's the 5kb insert? - Vector and insert are pretty much same size.. and it's not workin in a ' (reply: 3)
1045. efficiency of DNA cleanup columns - (reply: 8)
1046. amp v kan/neo resistance query - (reply: 3)
1047. Touch down PCR - (reply: 8)
1048. Large deletion mutants - Cloning (reply: 4)
1049. How can I make a point mutation - (reply: 4)
1050. Inserting tag into the vector. - where is IRES? (reply: 11)
1022. cut-delete-insert once more - just cloning? - (reply: 3)
1023. inclusion of translation start site when cloning promoter insert - (reply: 3)
1024. digest, blunt end, religate, digest.... - so many steps, maybe can skip purification... (reply: 2)
1025. cloning experimental design to ligate three PCR products into one plasmid - (reply: 4)
1026. possible contamination of TA cloning vector? - (reply: 4)
1027. Competant cells & incompetant scientist - No colonies formed (reply: 8)
1028. Explanation of DNA Precipitation - (reply: 4)
1029. How big can be the insert? - (reply: 1)
1030. electroporation cuvettes-single use only? - (reply: 9)
1031. After double digest my plasmid is 2Kbp shorter! - (reply: 3)
1032. Map of MIGR1 retroviral vector needed - (reply: 1)
1033. why competent cells take up only 1 type of plasmids? - (reply: 7)
1034. pXT7 vector map - (reply: 3)
1035. Ecoli genomic recombination causing cloning problems - after ligation vector size and sequence change (reply: 5)
1036. cloning of Poly A tail - (reply: 2)
1037. one blunt and one sticky ligation...something wrong - HELP!!! (reply: 2)
1038. help in cloning the promoter region of a gene - (reply: 3)
1039. wrong sequencing result?! - (reply: 13)
1040. Could anyone tell me where I can buy XL10-GOLD strain from? - (reply: 6)
1041. miniprep: no precipitate after PEG treatment. - (reply: 4)
1042. NAME OF SOB MEDIUM - Does anybody know where to find what "SOB" or "SOC" stands f (reply: 1)
1043. Cutting out of a T vector - Cutting sites in primers vs sites in vector (reply: 3)
1044. Ligation problem, might be bc/ it's the 5kb insert? - Vector and insert are pretty much same size.. and it's not workin in a ' (reply: 3)
1045. efficiency of DNA cleanup columns - (reply: 8)
1046. amp v kan/neo resistance query - (reply: 3)
1047. Touch down PCR - (reply: 8)
1048. Large deletion mutants - Cloning (reply: 4)
1049. How can I make a point mutation - (reply: 4)
1050. Inserting tag into the vector. - where is IRES? (reply: 11)