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121. colony screening by sequencing - (reply: 4)
122. question in ligation - (reply: 1)
123. Ligation problem - 2 Questions (reply: 5)
124. gateway question - pdonr221 (reply: 2)
125. Lil math question for you guys...very curious about answers - (reply: 5)
126. This is the gel pic that is confusing me and my boss - (reply: 7)
127. having difficulty in ligating 70bp dsDNA fragments - (reply: 4)
128. PEG 4000 for ligation - (reply: 5)
129. how can I clone a CYP450? - (reply: 1)
130. Wise expertise needed......Puzzled by something minor..concentration of annealed - (reply: 4)
131. pUC19 - cut sites (reply: 3)
132. Digestion and ligation - Do I omit the dephosphorylation steps? (reply: 4)
133. Help - problems ligating two large framents - Ligation of two large fragments (reply: 3)
134. How long do I incubate after transformation? ? - (reply: 7)
135. 3 way Ligation Issues... - (reply: 11)
136. Problem with transformation - (reply: 4)
137. Problems with site-directed mutagenesis - (reply: 2)
138. Combinations of SalI and HindIII - Conditions for double digestion (reply: 4)
139. It keeps getting better and better lol my ligase might be out to get me - (reply: 2)
140. whats the deal with satellite colonies? - (reply: 1)
141. 5 inserts, 1 colony..dreaming of a better cloning world - (reply: 6)
142. chromosomal DNA extraction E. coli - (reply: 3)
143. PCR cloning - (reply: 5)
144. How to conduct DNA cloning if plasmid's restriciton site is un-unique - (reply: 5)
145. how many plasmids can a competent bacteria take - (reply: 9)
146. band in -ve rtPCR - (reply: 2)
147. Star activity in BamHI - How severe a problem is this? (reply: 5)
148. Cloning of 3 kb fragment - (reply: 5)
149. GE health-maxiprep - (reply: 3)
150. pBLuescript transformation - (reply: 1)
122. question in ligation - (reply: 1)
123. Ligation problem - 2 Questions (reply: 5)
124. gateway question - pdonr221 (reply: 2)
125. Lil math question for you guys...very curious about answers - (reply: 5)
126. This is the gel pic that is confusing me and my boss - (reply: 7)
127. having difficulty in ligating 70bp dsDNA fragments - (reply: 4)
128. PEG 4000 for ligation - (reply: 5)
129. how can I clone a CYP450? - (reply: 1)
130. Wise expertise needed......Puzzled by something minor..concentration of annealed - (reply: 4)
131. pUC19 - cut sites (reply: 3)
132. Digestion and ligation - Do I omit the dephosphorylation steps? (reply: 4)
133. Help - problems ligating two large framents - Ligation of two large fragments (reply: 3)
134. How long do I incubate after transformation? ? - (reply: 7)
135. 3 way Ligation Issues... - (reply: 11)
136. Problem with transformation - (reply: 4)
137. Problems with site-directed mutagenesis - (reply: 2)
138. Combinations of SalI and HindIII - Conditions for double digestion (reply: 4)
139. It keeps getting better and better lol my ligase might be out to get me - (reply: 2)
140. whats the deal with satellite colonies? - (reply: 1)
141. 5 inserts, 1 colony..dreaming of a better cloning world - (reply: 6)
142. chromosomal DNA extraction E. coli - (reply: 3)
143. PCR cloning - (reply: 5)
144. How to conduct DNA cloning if plasmid's restriciton site is un-unique - (reply: 5)
145. how many plasmids can a competent bacteria take - (reply: 9)
146. band in -ve rtPCR - (reply: 2)
147. Star activity in BamHI - How severe a problem is this? (reply: 5)
148. Cloning of 3 kb fragment - (reply: 5)
149. GE health-maxiprep - (reply: 3)
150. pBLuescript transformation - (reply: 1)