growth time of bacteria after transform - (Jun/30/2008 )

hello

i'v inserted a gene fragment (1428bp) into EGFPC1 plasmid that is a transcription factor of mouse . when i transformed it into 2 kinds of bacterium , their growth time was changed from 18h to 48h.


now, my concern is that why such thing happened.

Thanks very much

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If you can figure that our, you can write a separate paper! It is interesting. See if this factor is an ancient conserved one (to prokaryotes-unlikely, but..), if there is any domain that has some relevant activities etc. See if this is same with yeast replication/growth and mammalian cell line rep/growth times. And you have a great study on your hands!

Otherwise, use that additional 30 hours to check out bars in your area.

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Toxic gene maybe?

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