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1501. BamHI/XbaI cloning, bad combination? - (reply: 3)
1502. Problems with STBL3 cells - Digesting DNA from STBL3 cells (reply: 7)
1503. broad spectrum transposon for gram positive bacteria - (reply: 2)
1504. Has anyone used In-Fusion Cloning Kit? - (reply: 3)
1505. cloning of big insert(6.4Kb) in pUC18/19 - (reply: 2)
1506. How to exchange CMV for another promoter - (reply: 2)
1507. is the polylinker in pUC18 plasmid a artificially inserted fragment? - want to know whether the polylinker site is a inserted or naturally present in t (reply: 1)
1508. Subcloing PCR fragments - (reply: 1)
1509. Why I keep getting no colonies from transformation of PCR products - help! (reply: 5)
1510. AMBION RLM-RACE Kit - I need to do some 5' RACE, is Ambion's the best kit? (reply: 1)
1511. Expression problem in pQE30 vector system - (reply: 2)
1512. Ligation/Transformation problem - (reply: 4)
1513. pcDNA 3.1/pTarget and stable cell line - (reply: 10)
1514. vector with tag recommendation - (reply: 10)
1515. Vector databases and resources - (reply: 5)
1516. Topo-Cloning over 3 kb - (reply: 5)
1517. why the inserted fragment can not be cutted ..... - the inserted fragment can not cutted by the enzymes used (reply: 1)
1518. need help,why colony pcr of my blue has product what i need - (reply: 2)
1519. Double Digestion and Single Digestion same result? - Confusion about Plasmid and Insert alignment (reply: 4)
1520. Types of DNA polymerases that you use. - (reply: 3)
1521. so lost! stupid quenstion about primers.... - designing the primers (reply: 2)
1522. Question about Nox gene - (reply: 1)
1523. designing the sequencing primer - (reply: 2)
1524. Mutagenic PCR unsuccessful :( - pls help.... (reply: 7)
1525. Me again...on type of polymerase and REs I should use... - (reply: 6)
1526. design of PRC primers of full ORF - (reply: 3)
1527. Cloning - how to avoid religation and no insert? - (reply: 13)
1528. RT-PCR with an old DNaseI? - (reply: 3)
1529. deletion during cloning - (reply: 3)
1530. Transformation - (reply: 4)
1502. Problems with STBL3 cells - Digesting DNA from STBL3 cells (reply: 7)
1503. broad spectrum transposon for gram positive bacteria - (reply: 2)
1504. Has anyone used In-Fusion Cloning Kit? - (reply: 3)
1505. cloning of big insert(6.4Kb) in pUC18/19 - (reply: 2)
1506. How to exchange CMV for another promoter - (reply: 2)
1507. is the polylinker in pUC18 plasmid a artificially inserted fragment? - want to know whether the polylinker site is a inserted or naturally present in t (reply: 1)
1508. Subcloing PCR fragments - (reply: 1)
1509. Why I keep getting no colonies from transformation of PCR products - help! (reply: 5)
1510. AMBION RLM-RACE Kit - I need to do some 5' RACE, is Ambion's the best kit? (reply: 1)
1511. Expression problem in pQE30 vector system - (reply: 2)
1512. Ligation/Transformation problem - (reply: 4)
1513. pcDNA 3.1/pTarget and stable cell line - (reply: 10)
1514. vector with tag recommendation - (reply: 10)
1515. Vector databases and resources - (reply: 5)
1516. Topo-Cloning over 3 kb - (reply: 5)
1517. why the inserted fragment can not be cutted ..... - the inserted fragment can not cutted by the enzymes used (reply: 1)
1518. need help,why colony pcr of my blue has product what i need - (reply: 2)
1519. Double Digestion and Single Digestion same result? - Confusion about Plasmid and Insert alignment (reply: 4)
1520. Types of DNA polymerases that you use. - (reply: 3)
1521. so lost! stupid quenstion about primers.... - designing the primers (reply: 2)
1522. Question about Nox gene - (reply: 1)
1523. designing the sequencing primer - (reply: 2)
1524. Mutagenic PCR unsuccessful :( - pls help.... (reply: 7)
1525. Me again...on type of polymerase and REs I should use... - (reply: 6)
1526. design of PRC primers of full ORF - (reply: 3)
1527. Cloning - how to avoid religation and no insert? - (reply: 13)
1528. RT-PCR with an old DNaseI? - (reply: 3)
1529. deletion during cloning - (reply: 3)
1530. Transformation - (reply: 4)