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481. cloning a 3kb fragment into a 6kb vector - or maybe an EcoRI/HindIII double digest problem? (reply: 3)
482. route map for cloning a gene - steps involved in subcloning a gene (reply: 3)
483. Cloning toxic gene - (reply: 2)
484. Cloning PCR product blunt + restriction site - (reply: 2)
485. gene targeting in ES cells - (reply: 1)
486. help in cloning 6kb pcr fragment into topo? - (reply: 5)
487. problem with cloning and sequencing. - (reply: 10)
488. PCR of lambda phage - (reply: 1)
489. How to identified stop codon? - (reply: 5)
490. Restriction digestion and DNA degradation problem - Restriction digestion and DNA degradation problem (reply: 1)
491. What EDTA does in annealing of DNA and vector? - (reply: 2)
492. Gene expression - (reply: 1)
493. prepare electrocompetent cells - (reply: 4)
494. Cloning into GATEWAY system - (reply: 3)
495. Need help with TOPO TA cloning problem! - TOPO TA clonig, ligation, no insert (reply: 23)
496. cloning in expression vector - (reply: 5)
497. Digest-Ligase-PCR Problems (T-DNA fingerprinting gone wrong) - After ligating digested product and doing PCR on it i get no DNA smear (reply: 2)
498. Linear plasmid for transfection - where should I cut the plasmid? (reply: 3)
499. Using single restriction enzyme SalI for cloning to a binary vector. How I will - Please its really very urgent...help me...help me.. (reply: 4)
500. Getting smear problems in plasmid run - (reply: 8)
501. Why Ligation and transformation not working? - (reply: 3)
502. T4 (without BSA) - How efficient the T4 is? (reply: 2)
503. DNA degradation while digestion. - (reply: 3)
504. why the difference in ligation temperature? - (reply: 1)
505. identifying genomic DNA contamination during plasmid isolation - (reply: 2)
506. Problems with certain restriction enzymes - on the verge of tears - (reply: 10)
507. PPARs expression in cardiac cells - (reply: 4)
508. E.coli promoterless lacZ vector - (reply: 2)
509. EL250 relectroporation - (reply: 4)
510. klenow/T4 question - Making one end blunt (reply: 4)
482. route map for cloning a gene - steps involved in subcloning a gene (reply: 3)
483. Cloning toxic gene - (reply: 2)
484. Cloning PCR product blunt + restriction site - (reply: 2)
485. gene targeting in ES cells - (reply: 1)
486. help in cloning 6kb pcr fragment into topo? - (reply: 5)
487. problem with cloning and sequencing. - (reply: 10)
488. PCR of lambda phage - (reply: 1)
489. How to identified stop codon? - (reply: 5)
490. Restriction digestion and DNA degradation problem - Restriction digestion and DNA degradation problem (reply: 1)
491. What EDTA does in annealing of DNA and vector? - (reply: 2)
492. Gene expression - (reply: 1)
493. prepare electrocompetent cells - (reply: 4)
494. Cloning into GATEWAY system - (reply: 3)
495. Need help with TOPO TA cloning problem! - TOPO TA clonig, ligation, no insert (reply: 23)
496. cloning in expression vector - (reply: 5)
497. Digest-Ligase-PCR Problems (T-DNA fingerprinting gone wrong) - After ligating digested product and doing PCR on it i get no DNA smear (reply: 2)
498. Linear plasmid for transfection - where should I cut the plasmid? (reply: 3)
499. Using single restriction enzyme SalI for cloning to a binary vector. How I will - Please its really very urgent...help me...help me.. (reply: 4)
500. Getting smear problems in plasmid run - (reply: 8)
501. Why Ligation and transformation not working? - (reply: 3)
502. T4 (without BSA) - How efficient the T4 is? (reply: 2)
503. DNA degradation while digestion. - (reply: 3)
504. why the difference in ligation temperature? - (reply: 1)
505. identifying genomic DNA contamination during plasmid isolation - (reply: 2)
506. Problems with certain restriction enzymes - on the verge of tears - (reply: 10)
507. PPARs expression in cardiac cells - (reply: 4)
508. E.coli promoterless lacZ vector - (reply: 2)
509. EL250 relectroporation - (reply: 4)
510. klenow/T4 question - Making one end blunt (reply: 4)