1. my strategy of ligation - (reply: 5)
2. problems with subcloning - (reply: 4)
3. DNA purification - Touble with isolating PCGP plasmid (reply: 3)
4. How to isolate intron of particular gene - (reply: 2)
5. Cloning hell - (reply: 1)
6. Good vector for genomic library - (reply: 1)
7. T7 or SP6 - (reply: 1)
8. Cloning Help! - (reply: 6)
9. Digestion, clean up lead to fewer DNA! Help... - (reply: 4)
10. DNA degradation? - (reply: 2)
11. EGFP cloning and membrane protein - (reply: 1)
12. [Help] New vector construct problem - (reply: 4)
13. suitable vectors for 3' UTR Luciferase? - (reply: 2)
14. Vector NTI no longer free - (reply: 6)
15. Cloning bisulphite converted amplicon - Need help on getting more clones (reply: 4)
16. is kan light sensitive? - (reply: 2)
17. small transformant colonies - (reply: 1)
18. directional cloning problem - two-digested vector and insert - ligate - no colonies?? (reply: 2)
19. a gene instead an other.. - (reply: 1)
20. Got problem with maxiprep - (reply: 3)
21. selective preciptation of DNA according to size - (reply: 4)
22. dam methylation? - digestion problem (reply: 3)
23. how long does it take a colony inoculation to reach log phase? - (reply: 2)
24. Calculating transformation efficiency? - (reply: 1)
25. Calculating copy number? - (reply: 1)
26. Cloning into pGBKT7 vector - (reply: 4)
27. southern blotting problem with DIG - southern blotting problem with DIG (reply: 4)
28. pRARE2 vector sequence - (reply: 4)
29. Inverse PCR ligation - (reply: 1)
30. SapI enzyme - (reply: 3)

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