|
1321. very low 230/260 readings - (reply: 2)
1322. ligation question! - (reply: 2)
1323. PCR and DMSO - (reply: 2)
1324. hurry! how can I clone large DNA fragment - noboday answer? (reply: 1)
1325. PCR product dimer (not primer dimer!) - PCR (reply: 2)
1326. Taq/PFU polymerase A overhang - bad for blunt ending? (reply: 3)
1327. expired CIAP - wondering wheter expired CIAP is still working... (reply: 5)
1328. Plasmid PCR - (reply: 5)
1329. Unique restriction sites in two sequences - (reply: 3)
1330. No colonies in my ligation - (reply: 3)
1331. ligation trouble - (reply: 2)
1332. problems in subcloning PCR products for TA cloning - (reply: 4)
1333. problems in a double digestion with NheI and EcoRI - Cloning problem (reply: 13)
1334. website for identifying restriction sites in a gene - (reply: 6)
1335. problem with the MCS - I can't use it. (reply: 4)
1336. cloning membrane protein with 2 tags - (reply: 2)
1337. Dominant negative mutation: Tyr is usually mutate to ? - Site directed mutation (reply: 1)
1338. difference between condition 30c and 37c - when transformate DNA to E.coli... (reply: 7)
1339. Agarose gels and SB Buffer - (reply: 7)
1340. ligation problem - (reply: 1)
1341. cDNA library screening with 5' specific primer - (reply: 1)
1342. problem cloning 2.5 kb fragment - (reply: 2)
1343. sequencing a restriction fragment - (reply: 4)
1344. Site-Directed Mutagenesis- Doubt - Overlapping primers (reply: 3)
1345. Help me! No colonies! Problem with gel purification? - (reply: 5)
1346. Restriction Analysis - (reply: 1)
1347. I need help --- ligation troubleshooting - (reply: 9)
1348. ligation with sticky and blunt ends - (reply: 3)
1349. desalting/concentrating DNA - (reply: 8)
1350. ligation inhibitors - (reply: 2)
1322. ligation question! - (reply: 2)
1323. PCR and DMSO - (reply: 2)
1324. hurry! how can I clone large DNA fragment - noboday answer? (reply: 1)
1325. PCR product dimer (not primer dimer!) - PCR (reply: 2)
1326. Taq/PFU polymerase A overhang - bad for blunt ending? (reply: 3)
1327. expired CIAP - wondering wheter expired CIAP is still working... (reply: 5)
1328. Plasmid PCR - (reply: 5)
1329. Unique restriction sites in two sequences - (reply: 3)
1330. No colonies in my ligation - (reply: 3)
1331. ligation trouble - (reply: 2)
1332. problems in subcloning PCR products for TA cloning - (reply: 4)
1333. problems in a double digestion with NheI and EcoRI - Cloning problem (reply: 13)
1334. website for identifying restriction sites in a gene - (reply: 6)
1335. problem with the MCS - I can't use it. (reply: 4)
1336. cloning membrane protein with 2 tags - (reply: 2)
1337. Dominant negative mutation: Tyr is usually mutate to ? - Site directed mutation (reply: 1)
1338. difference between condition 30c and 37c - when transformate DNA to E.coli... (reply: 7)
1339. Agarose gels and SB Buffer - (reply: 7)
1340. ligation problem - (reply: 1)
1341. cDNA library screening with 5' specific primer - (reply: 1)
1342. problem cloning 2.5 kb fragment - (reply: 2)
1343. sequencing a restriction fragment - (reply: 4)
1344. Site-Directed Mutagenesis- Doubt - Overlapping primers (reply: 3)
1345. Help me! No colonies! Problem with gel purification? - (reply: 5)
1346. Restriction Analysis - (reply: 1)
1347. I need help --- ligation troubleshooting - (reply: 9)
1348. ligation with sticky and blunt ends - (reply: 3)
1349. desalting/concentrating DNA - (reply: 8)
1350. ligation inhibitors - (reply: 2)