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1231. Would u plz give me some suggestions? - (reply: 13)
1232. chromosome length - telomers (reply: 2)
1233. Hanahan Method for preparation of Competent cell - a little problem (reply: 2)
1234. Triple ligation with IRES - (reply: 2)
1235. no product after 5' Race - (reply: 3)
1236. blunt-end ligation problem - (reply: 3)
1237. question about start codons - (reply: 1)
1238. Tips for transformation - which volume of ligation product? purification before transformation? Quantity o (reply: 4)
1239. cloning in a long vector (~10kb) without sequence changes - (reply: 2)
1240. Role of PEG in ligation - (reply: 3)
1241. Which DNA marker is this? - (reply: 4)
1242. Basic Question Restriction Digest - (reply: 11)
1243. does phenol:chloroform extracted plasmid creats problem in Mammalian cell cultur - (reply: 5)
1244. Does the T4 ligase works perfectly well in the presence of TAE and EtBr? - (reply: 1)
1245. re-dephosphorylation of vector? - (reply: 3)
1246. Dephosphorylation nick problem - (reply: 2)
1247. cloning vectors - limited insert size (reply: 10)
1248. adverse effects of lyophilization on DNA - (reply: 1)
1249. TA cloning...why is there no plasmid for BLUE colonies? - (reply: 2)
1250. Blunt end ligation help - (reply: 2)
1251. double-digest cloning problem - cloning (reply: 4)
1252. expression in Rosetta pLysS - how to get a good expression level?? (reply: 1)
1253. IPTG induction - IS IPTG freeze stable? (reply: 4)
1254. No colony after ligation? - (reply: 3)
1255. ATCC - ATCC number (reply: 1)
1256. Need some help! - (reply: 7)
1257. TA ligation - (reply: 4)
1258. Can pfu Polymerase make a blunt end? - (reply: 3)
1259. Electrophoresis of Plasmid - (reply: 3)
1260. Ligation of multi-fragments? - Has anybody tried to ligate more than 02 fragments in a single experiment? (reply: 2)
1232. chromosome length - telomers (reply: 2)
1233. Hanahan Method for preparation of Competent cell - a little problem (reply: 2)
1234. Triple ligation with IRES - (reply: 2)
1235. no product after 5' Race - (reply: 3)
1236. blunt-end ligation problem - (reply: 3)
1237. question about start codons - (reply: 1)
1238. Tips for transformation - which volume of ligation product? purification before transformation? Quantity o (reply: 4)
1239. cloning in a long vector (~10kb) without sequence changes - (reply: 2)
1240. Role of PEG in ligation - (reply: 3)
1241. Which DNA marker is this? - (reply: 4)
1242. Basic Question Restriction Digest - (reply: 11)
1243. does phenol:chloroform extracted plasmid creats problem in Mammalian cell cultur - (reply: 5)
1244. Does the T4 ligase works perfectly well in the presence of TAE and EtBr? - (reply: 1)
1245. re-dephosphorylation of vector? - (reply: 3)
1246. Dephosphorylation nick problem - (reply: 2)
1247. cloning vectors - limited insert size (reply: 10)
1248. adverse effects of lyophilization on DNA - (reply: 1)
1249. TA cloning...why is there no plasmid for BLUE colonies? - (reply: 2)
1250. Blunt end ligation help - (reply: 2)
1251. double-digest cloning problem - cloning (reply: 4)
1252. expression in Rosetta pLysS - how to get a good expression level?? (reply: 1)
1253. IPTG induction - IS IPTG freeze stable? (reply: 4)
1254. No colony after ligation? - (reply: 3)
1255. ATCC - ATCC number (reply: 1)
1256. Need some help! - (reply: 7)
1257. TA ligation - (reply: 4)
1258. Can pfu Polymerase make a blunt end? - (reply: 3)
1259. Electrophoresis of Plasmid - (reply: 3)
1260. Ligation of multi-fragments? - Has anybody tried to ligate more than 02 fragments in a single experiment? (reply: 2)