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691. Where exactly does a restriction enzyme cut in its recognition site? - (reply: 1)
692. plasmid cloning in E.coli - (reply: 2)
693. cloning problem - (reply: 1)
694. what is the plasmid nomenclature: eg.what does it mean by pUC, pCR, and other ve - (reply: 13)
695. Trouble sequencing DNA from mutagenesis reaction - (reply: 1)
696. How can I delete a fragment that is not surrounded by restriction sites? - (reply: 3)
697. Lots of colonies but they're all blue! - Do I need to adjust ligation ratios? (reply: 11)
698. Cloning large fragment - Cloning (reply: 2)
699. Low insert and vector concentration - (reply: 7)
700. Transformation efficiency? - (reply: 1)
701. trouble cloning into red vector - (reply: 1)
702. Problem in cloning lacZ in pUC18.lac- - ligation (reply: 2)
703. Cloning of synthetic oligos - how much oligos for ligation? (reply: 3)
704. how to remove biotin from DNA - (reply: 3)
705. How to grow frozen DB 3.1 cells - (reply: 1)
706. Induction of LacZ without IPTG - (reply: 2)
707. DNase treat my RNA prior to Generacer exp? - (reply: 1)
708. Religation again and again - Ligation problem (reply: 3)
709. "Ligate" two overlapping pcr products with PCR fusion? - (reply: 1)
710. help with moving an insert to a new vector - (reply: 4)
711. Screening PCR - DNA in wells? (reply: 1)
712. How to remove a few bases from my cloned insert using PCR - (reply: 3)
713. Purchase or make competent cells? - (reply: 8)
714. Ligation/ transforamtion troubles - (reply: 2)
715. Point Mutation in DNA - Quik change mutagenesis (reply: 1)
716. Blunt end cloning with Pfu and EcoRv - (reply: 3)
717. short DS-oligo cloning - (reply: 2)
718. size of colonies - (reply: 4)
719. Retroviral vector and recombination - Are Retroviral vector plasmids susceptible to recombination?why? (reply: 1)
720. Problem in cloning with electroporation. - (reply: 4)
692. plasmid cloning in E.coli - (reply: 2)
693. cloning problem - (reply: 1)
694. what is the plasmid nomenclature: eg.what does it mean by pUC, pCR, and other ve - (reply: 13)
695. Trouble sequencing DNA from mutagenesis reaction - (reply: 1)
696. How can I delete a fragment that is not surrounded by restriction sites? - (reply: 3)
697. Lots of colonies but they're all blue! - Do I need to adjust ligation ratios? (reply: 11)
698. Cloning large fragment - Cloning (reply: 2)
699. Low insert and vector concentration - (reply: 7)
700. Transformation efficiency? - (reply: 1)
701. trouble cloning into red vector - (reply: 1)
702. Problem in cloning lacZ in pUC18.lac- - ligation (reply: 2)
703. Cloning of synthetic oligos - how much oligos for ligation? (reply: 3)
704. how to remove biotin from DNA - (reply: 3)
705. How to grow frozen DB 3.1 cells - (reply: 1)
706. Induction of LacZ without IPTG - (reply: 2)
707. DNase treat my RNA prior to Generacer exp? - (reply: 1)
708. Religation again and again - Ligation problem (reply: 3)
709. "Ligate" two overlapping pcr products with PCR fusion? - (reply: 1)
710. help with moving an insert to a new vector - (reply: 4)
711. Screening PCR - DNA in wells? (reply: 1)
712. How to remove a few bases from my cloned insert using PCR - (reply: 3)
713. Purchase or make competent cells? - (reply: 8)
714. Ligation/ transforamtion troubles - (reply: 2)
715. Point Mutation in DNA - Quik change mutagenesis (reply: 1)
716. Blunt end cloning with Pfu and EcoRv - (reply: 3)
717. short DS-oligo cloning - (reply: 2)
718. size of colonies - (reply: 4)
719. Retroviral vector and recombination - Are Retroviral vector plasmids susceptible to recombination?why? (reply: 1)
720. Problem in cloning with electroporation. - (reply: 4)