|
751. difficult cloning of large insert - (reply: 3)
752. DNA stuck in wells - (reply: 9)
753. F1 origin is it mandatory for a plasmid - (reply: 3)
754. Free program for primer designing? - Need to check promoter sequence for possible primer binding sites (reply: 3)
755. Double digest question for ligation - (reply: 4)
756. cloning artifact? - (reply: 4)
757. XbaI and e-coli strains DH5α and XL-1 Blue - RE XbaI problem (reply: 2)
758. how many colonies to screen? - (reply: 5)
759. can anyone help me to explain it? - (reply: 4)
760. PNK treating insert? - (reply: 2)
761. E.coli plates question - (reply: 2)
762. Problem in dephosphorylation - (reply: 7)
763. Ligation reaction gel picture - (reply: 1)
764. Destruction of restriction site - (reply: 1)
765. DNA storage: TE vs water - (reply: 1)
766. TA-cloning vector contamination - (reply: 3)
767. Cells grow on Amp plates but not in LB with Amp - Amp problem, bug strain problem, or ligation problem ? or all of the a (reply: 7)
768. Vector with two promoters - Molecular Biology (reply: 2)
769. designing primers for cloning - designing primers for cloning (reply: 1)
770. Contamination from satellite colonies - (reply: 1)
771. did NEBiolab change BamH1's buffer to buffer 3? - (reply: 2)
772. Transformation-Min plasmid reqd - (reply: 3)
773. should I go on to the ligation? urgent - (reply: 4)
774. Spec-ed/Loaded DNA inconsistency after digestion of mini-preps - (reply: 2)
775. Insert being cut by E. coli? - (reply: 2)
776. Overview of restriction enzyme activity... - ...depending on number of bases flanking? (reply: 2)
777. PLASMID DNA yield - (reply: 3)
778. blunt end ligation problem - (reply: 4)
779. Protecting against Bgl II digestion - (reply: 2)
780. About Reverse transcriptase PCR followed by PCR - primers (reply: 1)
752. DNA stuck in wells - (reply: 9)
753. F1 origin is it mandatory for a plasmid - (reply: 3)
754. Free program for primer designing? - Need to check promoter sequence for possible primer binding sites (reply: 3)
755. Double digest question for ligation - (reply: 4)
756. cloning artifact? - (reply: 4)
757. XbaI and e-coli strains DH5α and XL-1 Blue - RE XbaI problem (reply: 2)
758. how many colonies to screen? - (reply: 5)
759. can anyone help me to explain it? - (reply: 4)
760. PNK treating insert? - (reply: 2)
761. E.coli plates question - (reply: 2)
762. Problem in dephosphorylation - (reply: 7)
763. Ligation reaction gel picture - (reply: 1)
764. Destruction of restriction site - (reply: 1)
765. DNA storage: TE vs water - (reply: 1)
766. TA-cloning vector contamination - (reply: 3)
767. Cells grow on Amp plates but not in LB with Amp - Amp problem, bug strain problem, or ligation problem ? or all of the a (reply: 7)
768. Vector with two promoters - Molecular Biology (reply: 2)
769. designing primers for cloning - designing primers for cloning (reply: 1)
770. Contamination from satellite colonies - (reply: 1)
771. did NEBiolab change BamH1's buffer to buffer 3? - (reply: 2)
772. Transformation-Min plasmid reqd - (reply: 3)
773. should I go on to the ligation? urgent - (reply: 4)
774. Spec-ed/Loaded DNA inconsistency after digestion of mini-preps - (reply: 2)
775. Insert being cut by E. coli? - (reply: 2)
776. Overview of restriction enzyme activity... - ...depending on number of bases flanking? (reply: 2)
777. PLASMID DNA yield - (reply: 3)
778. blunt end ligation problem - (reply: 4)
779. Protecting against Bgl II digestion - (reply: 2)
780. About Reverse transcriptase PCR followed by PCR - primers (reply: 1)