|
841. how to ligate random oligos to vector to construct a library? - (reply: 6)
842. dna staining dyes - which DNA staining dyes do you use (reply: 4)
843. expression construct need the addition of STOP Codon? - (reply: 2)
844. Is it posible to make ligation with DNA digested and stored at -20 - (reply: 2)
845. overlap extension using PCR (3way PCR) - (reply: 1)
846. help with recircularizing a blunt-ended vector? - (reply: 6)
847. Fusion PCR for eventual TA cloning - (reply: 2)
848. How design a linker - (reply: 1)
849. if you plate 40 ul of your electrocomp cells only... - would you get any colonies? (reply: 13)
850. Ligation insert without PCR? - (reply: 6)
851. Which antibiotic to choose? - puro vs hygro vs zeo etc (reply: 4)
852. long size cDNA cloning - (reply: 1)
853. How many ligase units? - probably a very stupid question... (reply: 4)
854. Transformation problems - time of transformation very low (0.7 ms) ???? (reply: 7)
855. Which enzyme is to be digested first in sequential RE? - In vector preparation for cloning... (reply: 7)
856. Help with Colony PCR - Tips and Tricks (reply: 11)
857. Restriction site - (reply: 3)
858. Mutation - (reply: 7)
859. cloning size - plasmid (reply: 2)
860. Help with Cloning into LARGE vector - (reply: 11)
861. double digestion with BamHI and HindIII - (reply: 13)
862. why does ligase buffer precipitate? - is it ok just to vortex it?? (reply: 12)
863. How to solve the problem of toxic ligation? - Are there any other competent cells better than DH5a dealing with toxi (reply: 3)
864. Help w/ CLONING...lots of transformants but no insert... - (reply: 8)
865. Eco47III is it problematic? - Selfligation doesnot work with Eco47III... (reply: 10)
866. volume of ligation product - (reply: 6)
867. plasmid stability - (reply: 4)
868. southern blot probe design - (reply: 2)
869. cloning large inserts in large vectors - (reply: 4)
870. 2 plasmids in one competent cell - is this possible? - Cloning query (reply: 9)
842. dna staining dyes - which DNA staining dyes do you use (reply: 4)
843. expression construct need the addition of STOP Codon? - (reply: 2)
844. Is it posible to make ligation with DNA digested and stored at -20 - (reply: 2)
845. overlap extension using PCR (3way PCR) - (reply: 1)
846. help with recircularizing a blunt-ended vector? - (reply: 6)
847. Fusion PCR for eventual TA cloning - (reply: 2)
848. How design a linker - (reply: 1)
849. if you plate 40 ul of your electrocomp cells only... - would you get any colonies? (reply: 13)
850. Ligation insert without PCR? - (reply: 6)
851. Which antibiotic to choose? - puro vs hygro vs zeo etc (reply: 4)
852. long size cDNA cloning - (reply: 1)
853. How many ligase units? - probably a very stupid question... (reply: 4)
854. Transformation problems - time of transformation very low (0.7 ms) ???? (reply: 7)
855. Which enzyme is to be digested first in sequential RE? - In vector preparation for cloning... (reply: 7)
856. Help with Colony PCR - Tips and Tricks (reply: 11)
857. Restriction site - (reply: 3)
858. Mutation - (reply: 7)
859. cloning size - plasmid (reply: 2)
860. Help with Cloning into LARGE vector - (reply: 11)
861. double digestion with BamHI and HindIII - (reply: 13)
862. why does ligase buffer precipitate? - is it ok just to vortex it?? (reply: 12)
863. How to solve the problem of toxic ligation? - Are there any other competent cells better than DH5a dealing with toxi (reply: 3)
864. Help w/ CLONING...lots of transformants but no insert... - (reply: 8)
865. Eco47III is it problematic? - Selfligation doesnot work with Eco47III... (reply: 10)
866. volume of ligation product - (reply: 6)
867. plasmid stability - (reply: 4)
868. southern blot probe design - (reply: 2)
869. cloning large inserts in large vectors - (reply: 4)
870. 2 plasmids in one competent cell - is this possible? - Cloning query (reply: 9)