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31. repeting PCR failing the reaction - (reply: 2)
32. Kanamycin resistance gene in Gram-positive bacteria - (reply: 2)
33. PCR fragment ligation - ligation of 3 pcr frangments (reply: 1)
34. Transformation Dilemma - I hope if you can figure out (reply: 5)
35. ligation and transformation problems - (wrong) oligos (reply: 4)
36. transformation of Saccharomyces cerevisiae - (reply: 1)
37. Growth of E.Coli culture - please help out-i am desperate (reply: 11)
38. phrGFP-1 - searching for vector sequence - (reply: 6)
39. 3 piece ligation - (reply: 4)
40. Determing the size of a cDNA library - (reply: 2)
41. transformation after miniprep - (reply: 7)
42. Unique cohesive overhangs cloning + cohesive/blunt ends cloning - molar ratio / use of PEG in cohesive-blunt ligation (reply: 8)
43. Genome Bank - How to construct a genome bank (reply: 4)
44. "ladder" ligation result - cloning is the bane of my existance (reply: 18)
45. before electro-transformation, remove T4 Ligase or not? - (reply: 3)
46. funny result from pGEX-insert cloned in DH5 alpha - insert is the wrong size! (reply: 2)
47. Easiest cloning strategy for deleting part of plasmid using adapters? - (reply: 1)
48. Plasmid Isolation - (reply: 1)
49. RT-PCR primer design - RT-PCR primer design...need ur help (reply: 1)
50. HOW to clone some kinases? - clone CDK2,CDK3,MAPK14 from HeLa (reply: 2)
51. problem in cloning, if any one can help me out - (reply: 2)
52. Transformation failure - (reply: 1)
53. Too many colonies - Too many colonies (reply: 1)
54. Sequential digestion protocol - (reply: 3)
55. TOPO® TA cloning - (reply: 3)
56. non dephosphorylated vector gave white colonies - (reply: 2)
57. Subcloning and Mutations - (reply: 1)
58. shRNA ligation problem - (reply: 3)
59. terrific broth mistake? - (reply: 1)
60. pUC19 ligation - (reply: 1)
32. Kanamycin resistance gene in Gram-positive bacteria - (reply: 2)
33. PCR fragment ligation - ligation of 3 pcr frangments (reply: 1)
34. Transformation Dilemma - I hope if you can figure out (reply: 5)
35. ligation and transformation problems - (wrong) oligos (reply: 4)
36. transformation of Saccharomyces cerevisiae - (reply: 1)
37. Growth of E.Coli culture - please help out-i am desperate (reply: 11)
38. phrGFP-1 - searching for vector sequence - (reply: 6)
39. 3 piece ligation - (reply: 4)
40. Determing the size of a cDNA library - (reply: 2)
41. transformation after miniprep - (reply: 7)
42. Unique cohesive overhangs cloning + cohesive/blunt ends cloning - molar ratio / use of PEG in cohesive-blunt ligation (reply: 8)
43. Genome Bank - How to construct a genome bank (reply: 4)
44. "ladder" ligation result - cloning is the bane of my existance (reply: 18)
45. before electro-transformation, remove T4 Ligase or not? - (reply: 3)
46. funny result from pGEX-insert cloned in DH5 alpha - insert is the wrong size! (reply: 2)
47. Easiest cloning strategy for deleting part of plasmid using adapters? - (reply: 1)
48. Plasmid Isolation - (reply: 1)
49. RT-PCR primer design - RT-PCR primer design...need ur help (reply: 1)
50. HOW to clone some kinases? - clone CDK2,CDK3,MAPK14 from HeLa (reply: 2)
51. problem in cloning, if any one can help me out - (reply: 2)
52. Transformation failure - (reply: 1)
53. Too many colonies - Too many colonies (reply: 1)
54. Sequential digestion protocol - (reply: 3)
55. TOPO® TA cloning - (reply: 3)
56. non dephosphorylated vector gave white colonies - (reply: 2)
57. Subcloning and Mutations - (reply: 1)
58. shRNA ligation problem - (reply: 3)
59. terrific broth mistake? - (reply: 1)
60. pUC19 ligation - (reply: 1)