|
1651. PQE vector - Getting protein in its soluble form (reply: 3)
1652. blue/white screening - problem with recombinant colonies (reply: 1)
1653. cDNA cloning problem - No product in PCR (reply: 5)
1654. Calculate insert/vector ratio for ligation - (reply: 8)
1655. Ligation reaction - temperature and duration consideration - (reply: 5)
1656. Directional cloning and clone mutations - each clone has a different mutation! (reply: 1)
1657. double digestion - Time for digestion (reply: 1)
1658. Cloning issue - vector insert ratio - (reply: 3)
1659. ligation/transformation issue - (reply: 5)
1660. one end blunt end and other end sticky end-- ligation - vector with one end sticky and other blunt end (reply: 13)
1661. subcloning fragments - cloning (reply: 5)
1662. cloning large gene - advice (reply: 2)
1663. help:failure of extraction of plasmid DNA - (reply: 2)
1664. Expression problems with pIRES vector from Clontech - EGFP at MCSB site cannot be expressed! (reply: 1)
1665. Cloning - Electrocompetent vs chemically competent (reply: 1)
1666. Transformation problem (HELP!) - (reply: 4)
1667. SV40-less Luciferase promoter plasmid? - (reply: 2)
1668. Help on ligation of big fragment(9kb). - ideas on good ligases? (reply: 2)
1669. Validity of sequences via TA cloning - (reply: 1)
1670. QIagen maxi prep - urgent....please help (reply: 4)
1671. Promoter insertion in pUAST vector - Promoter insertion in pUAST vector (reply: 1)
1672. cannot isolate plasmid from mutants - please HELP! (reply: 1)
1673. cloning problems - cloning problems (reply: 8)
1674. Ligation- what to do if DNA is not concentrated? - (reply: 7)
1675. Sticky ends to blunt - (reply: 5)
1676. In frame stop codon! - (reply: 1)
1677. quick cloning question - (reply: 3)
1678. Is it possible to gel purify the ligated product? - Avoid non cut vector or self-liagted vector contam (reply: 2)
1679. double digest not working? - BamHI+SacI double digest (reply: 4)
1680. problems cloning into plenti6/V5 lentiviral vector - (reply: 2)
1652. blue/white screening - problem with recombinant colonies (reply: 1)
1653. cDNA cloning problem - No product in PCR (reply: 5)
1654. Calculate insert/vector ratio for ligation - (reply: 8)
1655. Ligation reaction - temperature and duration consideration - (reply: 5)
1656. Directional cloning and clone mutations - each clone has a different mutation! (reply: 1)
1657. double digestion - Time for digestion (reply: 1)
1658. Cloning issue - vector insert ratio - (reply: 3)
1659. ligation/transformation issue - (reply: 5)
1660. one end blunt end and other end sticky end-- ligation - vector with one end sticky and other blunt end (reply: 13)
1661. subcloning fragments - cloning (reply: 5)
1662. cloning large gene - advice (reply: 2)
1663. help:failure of extraction of plasmid DNA - (reply: 2)
1664. Expression problems with pIRES vector from Clontech - EGFP at MCSB site cannot be expressed! (reply: 1)
1665. Cloning - Electrocompetent vs chemically competent (reply: 1)
1666. Transformation problem (HELP!) - (reply: 4)
1667. SV40-less Luciferase promoter plasmid? - (reply: 2)
1668. Help on ligation of big fragment(9kb). - ideas on good ligases? (reply: 2)
1669. Validity of sequences via TA cloning - (reply: 1)
1670. QIagen maxi prep - urgent....please help (reply: 4)
1671. Promoter insertion in pUAST vector - Promoter insertion in pUAST vector (reply: 1)
1672. cannot isolate plasmid from mutants - please HELP! (reply: 1)
1673. cloning problems - cloning problems (reply: 8)
1674. Ligation- what to do if DNA is not concentrated? - (reply: 7)
1675. Sticky ends to blunt - (reply: 5)
1676. In frame stop codon! - (reply: 1)
1677. quick cloning question - (reply: 3)
1678. Is it possible to gel purify the ligated product? - Avoid non cut vector or self-liagted vector contam (reply: 2)
1679. double digest not working? - BamHI+SacI double digest (reply: 4)
1680. problems cloning into plenti6/V5 lentiviral vector - (reply: 2)