|
721. Blunt end cloning with Pfu and EcoRv - (reply: 3)
722. short DS-oligo cloning - (reply: 2)
723. size of colonies - (reply: 4)
724. Retroviral vector and recombination - Are Retroviral vector plasmids susceptible to recombination?why? (reply: 1)
725. Problem in cloning with electroporation. - (reply: 4)
726. digestion and ligation with BspH1 - digestion with BspH1 of insert , and Nco1 for the vector ,ligation pos (reply: 2)
727. Linear fragment uptake by E.coli - (reply: 2)
728. How to save my plasmid - I run out of my plasmid and my EColi doesn't grow (reply: 3)
729. Problem with Digestion or Ligation. How to tell? - (reply: 7)
730. urgent HincII digestion problem - (reply: 5)
731. TOPO cloning problem - (reply: 8)
732. subcloning help urgent! - (reply: 7)
733. BbVc1 - (reply: 1)
734. primers for promoter amplification - (reply: 1)
735. Looking for a protocol for a partial digest w/ NdeI - (reply: 3)
736. G418 selection protocol - need ideas for G418 selection (reply: 5)
737. Cloning after amplifying with Taq polymerase? - (reply: 5)
738. RT-PCR primer designing - (reply: 6)
739. difficult cloning of large insert - (reply: 3)
740. DNA stuck in wells - (reply: 9)
741. F1 origin is it mandatory for a plasmid - (reply: 3)
742. Free program for primer designing? - Need to check promoter sequence for possible primer binding sites (reply: 3)
743. Double digest question for ligation - (reply: 4)
744. cloning artifact? - (reply: 4)
745. XbaI and e-coli strains DH5α and XL-1 Blue - RE XbaI problem (reply: 2)
746. how many colonies to screen? - (reply: 5)
747. can anyone help me to explain it? - (reply: 4)
748. PNK treating insert? - (reply: 2)
749. E.coli plates question - (reply: 2)
750. Problem in dephosphorylation - (reply: 7)
722. short DS-oligo cloning - (reply: 2)
723. size of colonies - (reply: 4)
724. Retroviral vector and recombination - Are Retroviral vector plasmids susceptible to recombination?why? (reply: 1)
725. Problem in cloning with electroporation. - (reply: 4)
726. digestion and ligation with BspH1 - digestion with BspH1 of insert , and Nco1 for the vector ,ligation pos (reply: 2)
727. Linear fragment uptake by E.coli - (reply: 2)
728. How to save my plasmid - I run out of my plasmid and my EColi doesn't grow (reply: 3)
729. Problem with Digestion or Ligation. How to tell? - (reply: 7)
730. urgent HincII digestion problem - (reply: 5)
731. TOPO cloning problem - (reply: 8)
732. subcloning help urgent! - (reply: 7)
733. BbVc1 - (reply: 1)
734. primers for promoter amplification - (reply: 1)
735. Looking for a protocol for a partial digest w/ NdeI - (reply: 3)
736. G418 selection protocol - need ideas for G418 selection (reply: 5)
737. Cloning after amplifying with Taq polymerase? - (reply: 5)
738. RT-PCR primer designing - (reply: 6)
739. difficult cloning of large insert - (reply: 3)
740. DNA stuck in wells - (reply: 9)
741. F1 origin is it mandatory for a plasmid - (reply: 3)
742. Free program for primer designing? - Need to check promoter sequence for possible primer binding sites (reply: 3)
743. Double digest question for ligation - (reply: 4)
744. cloning artifact? - (reply: 4)
745. XbaI and e-coli strains DH5α and XL-1 Blue - RE XbaI problem (reply: 2)
746. how many colonies to screen? - (reply: 5)
747. can anyone help me to explain it? - (reply: 4)
748. PNK treating insert? - (reply: 2)
749. E.coli plates question - (reply: 2)
750. Problem in dephosphorylation - (reply: 7)