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1201. Why not a SINGLE RECOMBINANT ? - (reply: 11)
1202. how to clone blunt end and sticky end simultaneously? - (reply: 6)
1203. Chloramphenicol amplification - (reply: 5)
1204. Problem with transformation using electroporator from eppendorf - (reply: 7)
1205. single restriction enzyme digestion? - is it possible to insert PCR product? (reply: 17)
1206. Right Heat-pulse time in transformations - 30, 45, 90 or 3 min?? (reply: 4)
1207. How far 2 restriction sites should be? - how many bases in between for efficient digestion. (reply: 4)
1208. Double digest: efficiency - what does 50% active in given buffer mean? (reply: 1)
1209. cesium chloride - (reply: 2)
1210. During DNA replication - (reply: 6)
1211. problems with NdeI restriction enzyme? - (reply: 2)
1212. is there a restriction about amount of vector in a ligation? - (reply: 14)
1213. pwzl sequencing - (reply: 3)
1214. Full and Partial Digestion - (reply: 3)
1215. To dephosphorylate the vector or not? - For cloning PCR product (reply: 6)
1216. Cloning PCR product - Any ideas? (reply: 3)
1217. smearing in bands after digestion? - Why does a smear appear after digestion? (reply: 4)
1218. PCR using long primers and two overlapping templates - PCR using long primers and two overlapping templates (reply: 2)
1219. Would u plz give me some suggestions? - (reply: 13)
1220. chromosome length - telomers (reply: 2)
1221. Hanahan Method for preparation of Competent cell - a little problem (reply: 2)
1222. Triple ligation with IRES - (reply: 2)
1223. no product after 5' Race - (reply: 3)
1224. blunt-end ligation problem - (reply: 3)
1225. question about start codons - (reply: 1)
1226. Tips for transformation - which volume of ligation product? purification before transformation? Quantity o (reply: 4)
1227. cloning in a long vector (~10kb) without sequence changes - (reply: 2)
1228. Role of PEG in ligation - (reply: 3)
1229. Which DNA marker is this? - (reply: 4)
1230. Basic Question Restriction Digest - (reply: 11)
1202. how to clone blunt end and sticky end simultaneously? - (reply: 6)
1203. Chloramphenicol amplification - (reply: 5)
1204. Problem with transformation using electroporator from eppendorf - (reply: 7)
1205. single restriction enzyme digestion? - is it possible to insert PCR product? (reply: 17)
1206. Right Heat-pulse time in transformations - 30, 45, 90 or 3 min?? (reply: 4)
1207. How far 2 restriction sites should be? - how many bases in between for efficient digestion. (reply: 4)
1208. Double digest: efficiency - what does 50% active in given buffer mean? (reply: 1)
1209. cesium chloride - (reply: 2)
1210. During DNA replication - (reply: 6)
1211. problems with NdeI restriction enzyme? - (reply: 2)
1212. is there a restriction about amount of vector in a ligation? - (reply: 14)
1213. pwzl sequencing - (reply: 3)
1214. Full and Partial Digestion - (reply: 3)
1215. To dephosphorylate the vector or not? - For cloning PCR product (reply: 6)
1216. Cloning PCR product - Any ideas? (reply: 3)
1217. smearing in bands after digestion? - Why does a smear appear after digestion? (reply: 4)
1218. PCR using long primers and two overlapping templates - PCR using long primers and two overlapping templates (reply: 2)
1219. Would u plz give me some suggestions? - (reply: 13)
1220. chromosome length - telomers (reply: 2)
1221. Hanahan Method for preparation of Competent cell - a little problem (reply: 2)
1222. Triple ligation with IRES - (reply: 2)
1223. no product after 5' Race - (reply: 3)
1224. blunt-end ligation problem - (reply: 3)
1225. question about start codons - (reply: 1)
1226. Tips for transformation - which volume of ligation product? purification before transformation? Quantity o (reply: 4)
1227. cloning in a long vector (~10kb) without sequence changes - (reply: 2)
1228. Role of PEG in ligation - (reply: 3)
1229. Which DNA marker is this? - (reply: 4)
1230. Basic Question Restriction Digest - (reply: 11)