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1171. Problems (unspecific products) with PCR to determine the size of cDNA inserts - (reply: 7)
1172. Please help! Can Dh5alpha Competent cells be used for electroporation? - (reply: 8)
1173. Insert 2 single strand DNA into a vector - (reply: 6)
1174. Ligation failure using three DNA fragments. - Cohesive ligation failure (reply: 4)
1175. disappearing vector after digestion - (reply: 11)
1176. Expression of GFP fusion: splicing out of tag ? - (reply: 1)
1177. Lost in Fusion PCR - I cannot get any fusion product (reply: 1)
1178. shelf life of siRNA - (reply: 1)
1179. short protocols in molecular biology...... - maybe too short??? (reply: 16)
1180. Removing vector restriction sites - and replacing with new ones (reply: 8)
1181. how to ligate two ends with different enzyme site - (reply: 3)
1182. Is the insert not complete? - Sequencing Cloned PCR product (reply: 5)
1183. Problems about ligation - (reply: 4)
1184. what hybridization temp in pcr - (reply: 5)
1185. Pfx PCR problem - (reply: 6)
1186. No positive colony - Help (reply: 4)
1187. bleach pretreatment for RNA extraction - (reply: 1)
1188. Vector problems? - (reply: 7)
1189. Tip for transformation : give me your opinion please! - (reply: 8)
1190. Unable to obtain colonies from PCR-insert + vector ligation - (reply: 6)
1191. Gene synthesis - (reply: 1)
1192. Calculating insert/vector ratio - (reply: 7)
1193. double digestion problem - additional band after double digestion (reply: 2)
1194. signal peptide and transmembrane.. - (reply: 1)
1195. smear in PCR - No consistancy in amplification (reply: 3)
1196. klenow fill in protocol for making blunt end - (reply: 5)
1197. Cloning - Problems about cloning (reply: 2)
1198. Help : no positive colonies - (reply: 19)
1199. ligation - (reply: 1)
1200. insering cDNA before the tag.... - what problems should I consider? (reply: 17)
1172. Please help! Can Dh5alpha Competent cells be used for electroporation? - (reply: 8)
1173. Insert 2 single strand DNA into a vector - (reply: 6)
1174. Ligation failure using three DNA fragments. - Cohesive ligation failure (reply: 4)
1175. disappearing vector after digestion - (reply: 11)
1176. Expression of GFP fusion: splicing out of tag ? - (reply: 1)
1177. Lost in Fusion PCR - I cannot get any fusion product (reply: 1)
1178. shelf life of siRNA - (reply: 1)
1179. short protocols in molecular biology...... - maybe too short??? (reply: 16)
1180. Removing vector restriction sites - and replacing with new ones (reply: 8)
1181. how to ligate two ends with different enzyme site - (reply: 3)
1182. Is the insert not complete? - Sequencing Cloned PCR product (reply: 5)
1183. Problems about ligation - (reply: 4)
1184. what hybridization temp in pcr - (reply: 5)
1185. Pfx PCR problem - (reply: 6)
1186. No positive colony - Help (reply: 4)
1187. bleach pretreatment for RNA extraction - (reply: 1)
1188. Vector problems? - (reply: 7)
1189. Tip for transformation : give me your opinion please! - (reply: 8)
1190. Unable to obtain colonies from PCR-insert + vector ligation - (reply: 6)
1191. Gene synthesis - (reply: 1)
1192. Calculating insert/vector ratio - (reply: 7)
1193. double digestion problem - additional band after double digestion (reply: 2)
1194. signal peptide and transmembrane.. - (reply: 1)
1195. smear in PCR - No consistancy in amplification (reply: 3)
1196. klenow fill in protocol for making blunt end - (reply: 5)
1197. Cloning - Problems about cloning (reply: 2)
1198. Help : no positive colonies - (reply: 19)
1199. ligation - (reply: 1)
1200. insering cDNA before the tag.... - what problems should I consider? (reply: 17)