|
1621. T4 blunting follow by digestion - (reply: 2)
1622. Maxiprep Question - (reply: 16)
1623. Ligation at 16°C - problems wit ligation (reply: 6)
1624. maxiprep help - (reply: 12)
1625. Fast ligation - (reply: 8)
1626. Question on digestion - (reply: 4)
1627. seemingly easy ligations don't work - (reply: 11)
1628. M15 transformation problem? - (reply: 1)
1629. help pcr cloning problem - help pcr cloning problem!!!!!1 (reply: 2)
1630. Cloning in old days - (reply: 2)
1631. making more chemically competent cells - (reply: 6)
1632. in frame cloning - cloning (reply: 3)
1633. making competent cell - protocol molecular biology (reply: 8)
1634. pcr ligation - (reply: 2)
1635. double enzyme cut and ligation - (reply: 3)
1636. Ligation woes - ligation? what's wrong (reply: 2)
1637. Plasmid-self ligating - (reply: 6)
1638. A few questions about insertion into a plasmid - (reply: 7)
1639. Problem with directional cloning - Problem with directional cloning (reply: 8)
1640. ACK! Blunt end cloning - I'm confused.. - (reply: 2)
1641. phosphorylated oligos and vector - (reply: 2)
1642. QIAquick Gel Extration Kit - (reply: 8)
1643. pCGN sequence - (reply: 5)
1644. cloning - cloning in pet28a (reply: 2)
1645. Vector Construction- Tagging N-term - necessity of ATG in Gene of Interest (reply: 2)
1646. PQE vector - Getting protein in its soluble form (reply: 3)
1647. blue/white screening - problem with recombinant colonies (reply: 1)
1648. cDNA cloning problem - No product in PCR (reply: 5)
1649. Calculate insert/vector ratio for ligation - (reply: 8)
1650. Ligation reaction - temperature and duration consideration - (reply: 5)
1622. Maxiprep Question - (reply: 16)
1623. Ligation at 16°C - problems wit ligation (reply: 6)
1624. maxiprep help - (reply: 12)
1625. Fast ligation - (reply: 8)
1626. Question on digestion - (reply: 4)
1627. seemingly easy ligations don't work - (reply: 11)
1628. M15 transformation problem? - (reply: 1)
1629. help pcr cloning problem - help pcr cloning problem!!!!!1 (reply: 2)
1630. Cloning in old days - (reply: 2)
1631. making more chemically competent cells - (reply: 6)
1632. in frame cloning - cloning (reply: 3)
1633. making competent cell - protocol molecular biology (reply: 8)
1634. pcr ligation - (reply: 2)
1635. double enzyme cut and ligation - (reply: 3)
1636. Ligation woes - ligation? what's wrong (reply: 2)
1637. Plasmid-self ligating - (reply: 6)
1638. A few questions about insertion into a plasmid - (reply: 7)
1639. Problem with directional cloning - Problem with directional cloning (reply: 8)
1640. ACK! Blunt end cloning - I'm confused.. - (reply: 2)
1641. phosphorylated oligos and vector - (reply: 2)
1642. QIAquick Gel Extration Kit - (reply: 8)
1643. pCGN sequence - (reply: 5)
1644. cloning - cloning in pet28a (reply: 2)
1645. Vector Construction- Tagging N-term - necessity of ATG in Gene of Interest (reply: 2)
1646. PQE vector - Getting protein in its soluble form (reply: 3)
1647. blue/white screening - problem with recombinant colonies (reply: 1)
1648. cDNA cloning problem - No product in PCR (reply: 5)
1649. Calculate insert/vector ratio for ligation - (reply: 8)
1650. Ligation reaction - temperature and duration consideration - (reply: 5)