|
1411. Is cDNA a single or double stranded ? - (reply: 7)
1412. No band on double digestion of construct - (reply: 1)
1413. PCR with attB not working - (reply: 1)
1414. Very, VERY weird PCR results! - (reply: 4)
1415. does glycogen inhibit enzyme digestion or T4 ligation? - (reply: 11)
1416. why my ligation can not work - (reply: 2)
1417. BSA importance?/ Is Pac 1 a bad "cutter" - Use BSA? (reply: 2)
1418. How to elute plasmid spoted on paper - (reply: 3)
1419. stability of TOPO vector at RT - (reply: 3)
1420. PCR cloning problem - No colonies - (reply: 10)
1421. Transient transfection on a stable cell line - (reply: 6)
1422. pGBKT7 Yeast two hybrid - Problems while using pGBKT7 (reply: 1)
1423. pBluesript - (reply: 1)
1424. Huge problem in cloning, need help quite urgent - (reply: 2)
1425. Real time PCR - no product - (reply: 1)
1426. IPTG concentration - (reply: 7)
1427. does anyone have the pLSA-beta-geo vector map? - (reply: 6)
1428. plasmid rescue method - (reply: 3)
1429. the centrifuge speed to precipitate the 30bp oligo - (reply: 5)
1430. Blunt end cloning options - (reply: 1)
1431. "DNA molecular weight markers" paper - pQE-60 dig. with Dra1, BACSUB LDH with NCo1 (reply: 1)
1432. How should I check my colonies? - PCR check is not working...:( (reply: 5)
1433. basic RE digests acting weird - (reply: 3)
1434. question about mutagenic PCR. - (reply: 2)
1435. Very easy question. - (reply: 9)
1436. can you ligate gel slices directly? - (reply: 8)
1437. how to estimate amount of DNA using gel? - do u use agarose or acrylamide? (reply: 2)
1438. transformation step: 5min or 30min? - (reply: 10)
1439. Low melt temp agarose not melting - (reply: 13)
1440. tricky digestion for cloning gurus - (reply: 13)
1412. No band on double digestion of construct - (reply: 1)
1413. PCR with attB not working - (reply: 1)
1414. Very, VERY weird PCR results! - (reply: 4)
1415. does glycogen inhibit enzyme digestion or T4 ligation? - (reply: 11)
1416. why my ligation can not work - (reply: 2)
1417. BSA importance?/ Is Pac 1 a bad "cutter" - Use BSA? (reply: 2)
1418. How to elute plasmid spoted on paper - (reply: 3)
1419. stability of TOPO vector at RT - (reply: 3)
1420. PCR cloning problem - No colonies - (reply: 10)
1421. Transient transfection on a stable cell line - (reply: 6)
1422. pGBKT7 Yeast two hybrid - Problems while using pGBKT7 (reply: 1)
1423. pBluesript - (reply: 1)
1424. Huge problem in cloning, need help quite urgent - (reply: 2)
1425. Real time PCR - no product - (reply: 1)
1426. IPTG concentration - (reply: 7)
1427. does anyone have the pLSA-beta-geo vector map? - (reply: 6)
1428. plasmid rescue method - (reply: 3)
1429. the centrifuge speed to precipitate the 30bp oligo - (reply: 5)
1430. Blunt end cloning options - (reply: 1)
1431. "DNA molecular weight markers" paper - pQE-60 dig. with Dra1, BACSUB LDH with NCo1 (reply: 1)
1432. How should I check my colonies? - PCR check is not working...:( (reply: 5)
1433. basic RE digests acting weird - (reply: 3)
1434. question about mutagenic PCR. - (reply: 2)
1435. Very easy question. - (reply: 9)
1436. can you ligate gel slices directly? - (reply: 8)
1437. how to estimate amount of DNA using gel? - do u use agarose or acrylamide? (reply: 2)
1438. transformation step: 5min or 30min? - (reply: 10)
1439. Low melt temp agarose not melting - (reply: 13)
1440. tricky digestion for cloning gurus - (reply: 13)