|
391. Klenow - ligation problem - Can Klenow add some extra nucleotides.. (reply: 7)
392. QuickChnage Mutagenesis problem - no mutation, please help!!!!!!!! (reply: 14)
393. Tips on improving ligation+transformation from gel extraction - Let's contribute! (reply: 1)
394. DH5 keep kicking out my vector - (reply: 2)
395. sequence the same nucleotide - (reply: 2)
396. QIAprep vs. QIAquick column - (reply: 4)
397. problem with bi-enzyme cloning - (reply: 4)
398. Help with SOC medium contamination - (reply: 7)
399. Double ligation - What about cloning two fragments in the same reaction? (reply: 5)
400. genomic DNA contamination during plasmid preparation - (reply: 1)
401. human EF1alpha promoter sequence - human EF1alpha sequence (reply: 1)
402. Blunt-end/cohesive ends cloning - (reply: 7)
403. problem with transformation - ligation and cells seems to be fine (reply: 3)
404. puc19 vector - (reply: 1)
405. false positive in colony screening PCR? - (reply: 8)
406. SAP incubation time - how long do you incubate for?? (reply: 1)
407. Protein expression in E.coli DH5alpha? - (reply: 1)
408. pEGFP N1 vector - (reply: 6)
409. help trouble shooting: PstI/SapI cloning - Is SapI a nasty enzyme? (reply: 6)
410. MiniPrep - (reply: 3)
411. large plasmid extraction? - how? (reply: 5)
412. no plasmid extracted - low copy number - (reply: 2)
413. Help with TOPO TA cloning – too long or too many insert - (reply: 1)
414. Cloning big inserts - (reply: 4)
415. no GFP in cells - (reply: 7)
416. should I cut out signal peptide for expression - (reply: 1)
417. Random Amplification + TA cloning = HATE - (reply: 2)
418. how important a terminator is in cloning? - (reply: 3)
419. Storage of transformation reaction mix (Promega) - CLoning-transformation (reply: 2)
420. strange transformation products in subclone - (reply: 4)
392. QuickChnage Mutagenesis problem - no mutation, please help!!!!!!!! (reply: 14)
393. Tips on improving ligation+transformation from gel extraction - Let's contribute! (reply: 1)
394. DH5 keep kicking out my vector - (reply: 2)
395. sequence the same nucleotide - (reply: 2)
396. QIAprep vs. QIAquick column - (reply: 4)
397. problem with bi-enzyme cloning - (reply: 4)
398. Help with SOC medium contamination - (reply: 7)
399. Double ligation - What about cloning two fragments in the same reaction? (reply: 5)
400. genomic DNA contamination during plasmid preparation - (reply: 1)
401. human EF1alpha promoter sequence - human EF1alpha sequence (reply: 1)
402. Blunt-end/cohesive ends cloning - (reply: 7)
403. problem with transformation - ligation and cells seems to be fine (reply: 3)
404. puc19 vector - (reply: 1)
405. false positive in colony screening PCR? - (reply: 8)
406. SAP incubation time - how long do you incubate for?? (reply: 1)
407. Protein expression in E.coli DH5alpha? - (reply: 1)
408. pEGFP N1 vector - (reply: 6)
409. help trouble shooting: PstI/SapI cloning - Is SapI a nasty enzyme? (reply: 6)
410. MiniPrep - (reply: 3)
411. large plasmid extraction? - how? (reply: 5)
412. no plasmid extracted - low copy number - (reply: 2)
413. Help with TOPO TA cloning – too long or too many insert - (reply: 1)
414. Cloning big inserts - (reply: 4)
415. no GFP in cells - (reply: 7)
416. should I cut out signal peptide for expression - (reply: 1)
417. Random Amplification + TA cloning = HATE - (reply: 2)
418. how important a terminator is in cloning? - (reply: 3)
419. Storage of transformation reaction mix (Promega) - CLoning-transformation (reply: 2)
420. strange transformation products in subclone - (reply: 4)