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1261. Get stuck in a subcoloning: put 0.6kb into 7kb - (reply: 8)
1262. Making blunt and stick end vector - (reply: 2)
1263. No colony after ligation - (reply: 6)
1264. Ligation worked - maybe? - Aimikins - I've got colonies! Which size do I pick? (reply: 4)
1265. Cloning a PCR product after digestion - (reply: 7)
1266. Which Klenow product to use?! - With or without exo-nuclease activity? (reply: 5)
1267. troobleshoting for colonies background - (reply: 3)
1268. Cloning of long gene - (reply: 1)
1269. Silly question concening REs - (reply: 6)
1270. Cutting close to DNA ends - (reply: 6)
1271. Problem in picking colonies after transformation - (reply: 9)
1272. basal expression - Is it possible in DH5a or in Rosetta pLYS? - (reply: 3)
1273. Distinguish uncut vs. cut plasmids on agarose gel - (reply: 9)
1274. toxic gene ligation/transformation URGENT HELP! - (reply: 1)
1275. why dephosphoration of vector is not recommended? - (reply: 3)
1276. Is OVER-dephosphorylation possible? - (reply: 2)
1277. Positive and negative controls for transformation - What controls to use (reply: 4)
1278. Reusing Qiagen Tips - (reply: 2)
1279. Problems in cloning shRNA - (reply: 13)
1280. problem in ligation and transformation - (reply: 18)
1281. dimers of dna (not primers) following digest or pcr? - (reply: 11)
1282. Ethanol precipitation problem - (reply: 7)
1283. What amount of insert:vector should I use if I want a 3:1 molar ratio? - (reply: 2)
1284. cloning restriction fragment for sequencing - (reply: 1)
1285. Prepping and transformation - (reply: 2)
1286. How do I check frame? - (reply: 3)
1287. Cloning with real time PCR fragments - using SyberGreen from Applied Biosystem (reply: 3)
1288. Many colonies but no positive clone - (reply: 1)
1289. converting overhang into blunt end - can i use Pfu instead Klenow? (reply: 3)
1290. plasmid sequencing - (reply: 4)
1262. Making blunt and stick end vector - (reply: 2)
1263. No colony after ligation - (reply: 6)
1264. Ligation worked - maybe? - Aimikins - I've got colonies! Which size do I pick? (reply: 4)
1265. Cloning a PCR product after digestion - (reply: 7)
1266. Which Klenow product to use?! - With or without exo-nuclease activity? (reply: 5)
1267. troobleshoting for colonies background - (reply: 3)
1268. Cloning of long gene - (reply: 1)
1269. Silly question concening REs - (reply: 6)
1270. Cutting close to DNA ends - (reply: 6)
1271. Problem in picking colonies after transformation - (reply: 9)
1272. basal expression - Is it possible in DH5a or in Rosetta pLYS? - (reply: 3)
1273. Distinguish uncut vs. cut plasmids on agarose gel - (reply: 9)
1274. toxic gene ligation/transformation URGENT HELP! - (reply: 1)
1275. why dephosphoration of vector is not recommended? - (reply: 3)
1276. Is OVER-dephosphorylation possible? - (reply: 2)
1277. Positive and negative controls for transformation - What controls to use (reply: 4)
1278. Reusing Qiagen Tips - (reply: 2)
1279. Problems in cloning shRNA - (reply: 13)
1280. problem in ligation and transformation - (reply: 18)
1281. dimers of dna (not primers) following digest or pcr? - (reply: 11)
1282. Ethanol precipitation problem - (reply: 7)
1283. What amount of insert:vector should I use if I want a 3:1 molar ratio? - (reply: 2)
1284. cloning restriction fragment for sequencing - (reply: 1)
1285. Prepping and transformation - (reply: 2)
1286. How do I check frame? - (reply: 3)
1287. Cloning with real time PCR fragments - using SyberGreen from Applied Biosystem (reply: 3)
1288. Many colonies but no positive clone - (reply: 1)
1289. converting overhang into blunt end - can i use Pfu instead Klenow? (reply: 3)
1290. plasmid sequencing - (reply: 4)