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301. Problem with bands of PCR products - (reply: 2)
302. Expression problem - (reply: 6)
303. ask help for site mutation - help (reply: 1)
304. always two bands in colony PCR - What the hell did another band come from? (reply: 4)
305. PCR contamination - (reply: 1)
306. RE Enzyme Aliquot Dates - Just from curiousity... (reply: 1)
307. RE cloning a 2.3kb fragment into a 4.7kb vector - (reply: 4)
308. PEG removing A overhang? - low transformation efficiency (reply: 2)
309. miniprep problem - (reply: 11)
310. How can I create a T vector for PCR product cloning - (reply: 2)
311. PGEM-T easy gel - partial digestion? - (reply: 2)
312. NEB Turbo Electrocompetent E. coli - Colonies visible 8 h after electroporation!!!!!! Cool (reply: 1)
313. Primer design: how do I add a restriction site to my primer? - (reply: 2)
314. RE of PCR Fragment - (reply: 2)
315. Miniprep DNA digestion smear - (reply: 15)
316. purification、ligation and transformation problems - (reply: 11)
317. Help: PCR clone problem - (reply: 2)
318. Help in Clone Expression - Trasformants grow on plate but not in medium (reply: 2)
319. luciferase vector construction - (reply: 3)
320. only one band after double enzyme digestion - (reply: 4)
321. Why there is a artifical intron in some plasmids? - (reply: 4)
322. TetR primer - (reply: 1)
323. problem with site mutation - (reply: 5)
324. amplificatin of bacterial total RNA - (reply: 1)
325. can anybody give me a protocol of how to make a shRNA constrct? - (reply: 1)
326. Restriction Enzyme-EcoR V come frozen !? - (reply: 6)
327. Please comment on this simple cloning strategy - (reply: 6)
328. ligation transformation.......which chemically competent cells are the best? - (reply: 3)
329. TOPO primer choice for sequencing - (reply: 3)
330. EcoR1-Pst1 digest for vector and insert - (reply: 3)
302. Expression problem - (reply: 6)
303. ask help for site mutation - help (reply: 1)
304. always two bands in colony PCR - What the hell did another band come from? (reply: 4)
305. PCR contamination - (reply: 1)
306. RE Enzyme Aliquot Dates - Just from curiousity... (reply: 1)
307. RE cloning a 2.3kb fragment into a 4.7kb vector - (reply: 4)
308. PEG removing A overhang? - low transformation efficiency (reply: 2)
309. miniprep problem - (reply: 11)
310. How can I create a T vector for PCR product cloning - (reply: 2)
311. PGEM-T easy gel - partial digestion? - (reply: 2)
312. NEB Turbo Electrocompetent E. coli - Colonies visible 8 h after electroporation!!!!!! Cool (reply: 1)
313. Primer design: how do I add a restriction site to my primer? - (reply: 2)
314. RE of PCR Fragment - (reply: 2)
315. Miniprep DNA digestion smear - (reply: 15)
316. purification、ligation and transformation problems - (reply: 11)
317. Help: PCR clone problem - (reply: 2)
318. Help in Clone Expression - Trasformants grow on plate but not in medium (reply: 2)
319. luciferase vector construction - (reply: 3)
320. only one band after double enzyme digestion - (reply: 4)
321. Why there is a artifical intron in some plasmids? - (reply: 4)
322. TetR primer - (reply: 1)
323. problem with site mutation - (reply: 5)
324. amplificatin of bacterial total RNA - (reply: 1)
325. can anybody give me a protocol of how to make a shRNA constrct? - (reply: 1)
326. Restriction Enzyme-EcoR V come frozen !? - (reply: 6)
327. Please comment on this simple cloning strategy - (reply: 6)
328. ligation transformation.......which chemically competent cells are the best? - (reply: 3)
329. TOPO primer choice for sequencing - (reply: 3)
330. EcoR1-Pst1 digest for vector and insert - (reply: 3)