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1141. plasmids smaller than empty vector - using expensive cells - still not working (reply: 1)
1142. pDBleu vector - restriction problems (reply: 1)
1143. checking colonies by vector primers.... - (reply: 7)
1144. Gateway vectors - (reply: 1)
1145. Pfu polymerase from promega - (reply: 4)
1146. trouble ligating fusion-PCR into vector - (reply: 6)
1147. Any body knows the characters of plasmid pINT007 - (reply: 4)
1148. Satellite colonies - (reply: 3)
1149. Turbo Competent Cells - (reply: 3)
1150. adding restriction sites to degenerate primers - (reply: 9)
1151. subclone help - (reply: 2)
1152. RNA isolation from locus ceruleus - (reply: 2)
1153. Standard vs High fidelity polymerase, please help - No results with high fidelity (reply: 5)
1154. Plasmid isolation - (reply: 2)
1155. complete ligation HELP?! - (reply: 1)
1156. leader sequence - (reply: 1)
1157. weird blunt-sticky ligation case - (reply: 2)
1158. Suggestions on Research design re: Cloning the promoter region and measure induc - Molecular genetics, protocol (reply: 7)
1159. is there a shelf-life of x-gal stock? - (reply: 1)
1160. got colonies but can't check them... - help!!! conference in 2 weeks!! (reply: 4)
1161. cloning primer and tag questions - first try (reply: 2)
1162. Creating E.coli constructs in yeast cells - If you are fed up with bacteria (reply: 3)
1163. Sense or antisense? How can I tell directionality of insert? - Trying to make an antisense riboprobe via in vitro transcription with DIG-UTP fo (reply: 1)
1164. Quick question: splitting TOPO cloning kit - (reply: 7)
1165. PCR analysis of clones - Can a gene specific primer pair amplify a fragment in the wrong direction? (reply: 1)
1166. RNase treatment - (reply: 9)
1167. blue/white colony screening - (reply: 2)
1168. insert to vector ratios for ligations - what do y'all do? (reply: 4)
1169. pro-PCR with only one primer... - to check directionality. (reply: 4)
1170. Usage of salmon sperm DNA in yeast transformation? - (reply: 3)
1142. pDBleu vector - restriction problems (reply: 1)
1143. checking colonies by vector primers.... - (reply: 7)
1144. Gateway vectors - (reply: 1)
1145. Pfu polymerase from promega - (reply: 4)
1146. trouble ligating fusion-PCR into vector - (reply: 6)
1147. Any body knows the characters of plasmid pINT007 - (reply: 4)
1148. Satellite colonies - (reply: 3)
1149. Turbo Competent Cells - (reply: 3)
1150. adding restriction sites to degenerate primers - (reply: 9)
1151. subclone help - (reply: 2)
1152. RNA isolation from locus ceruleus - (reply: 2)
1153. Standard vs High fidelity polymerase, please help - No results with high fidelity (reply: 5)
1154. Plasmid isolation - (reply: 2)
1155. complete ligation HELP?! - (reply: 1)
1156. leader sequence - (reply: 1)
1157. weird blunt-sticky ligation case - (reply: 2)
1158. Suggestions on Research design re: Cloning the promoter region and measure induc - Molecular genetics, protocol (reply: 7)
1159. is there a shelf-life of x-gal stock? - (reply: 1)
1160. got colonies but can't check them... - help!!! conference in 2 weeks!! (reply: 4)
1161. cloning primer and tag questions - first try (reply: 2)
1162. Creating E.coli constructs in yeast cells - If you are fed up with bacteria (reply: 3)
1163. Sense or antisense? How can I tell directionality of insert? - Trying to make an antisense riboprobe via in vitro transcription with DIG-UTP fo (reply: 1)
1164. Quick question: splitting TOPO cloning kit - (reply: 7)
1165. PCR analysis of clones - Can a gene specific primer pair amplify a fragment in the wrong direction? (reply: 1)
1166. RNase treatment - (reply: 9)
1167. blue/white colony screening - (reply: 2)
1168. insert to vector ratios for ligations - what do y'all do? (reply: 4)
1169. pro-PCR with only one primer... - to check directionality. (reply: 4)
1170. Usage of salmon sperm DNA in yeast transformation? - (reply: 3)