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1561. No positive clones on blue/white selection - (reply: 3)
1562. Can we purify Miniprep DNA and use for transfection? - (reply: 3)
1563. supercoiled control - (reply: 5)
1564. ligation to transformation - (reply: 1)
1565. Digesting PCR products and cleaning up digestions - a few quick questions (reply: 4)
1566. coagulation in SDSPAGE Sample buffer - SDS PAGE (reply: 1)
1567. Hybridation vs Normal PCR - (reply: 2)
1568. Duration of cell line transfected - (reply: 2)
1569. Use Zeocin to isolate Zeocin resistant clones - (reply: 2)
1570. Two same oris (origins of replications) in one plasmid - (reply: 1)
1571. Searching for a pQE40 - (reply: 2)
1572. Beta mercaptoethanol? - (reply: 2)
1573. Retroviral Vector won't digest ideas! - (reply: 1)
1574. Site directed mutagenesis GFP to RFP - (reply: 4)
1575. Kozak Sequences and Mammalian Expression Vectors - (reply: 2)
1576. kits for mrna isolation in india - mrna isolation (reply: 1)
1577. My competent cells are misguiding me - (reply: 3)
1578. Vector self ligation? - (reply: 1)
1579. cloning problems - help! (reply: 4)
1580. High Fidelity DNA Polymerase: how many mutations per every kb of amplified DNA o - (reply: 10)
1581. rabbit intestinal cDNA - Full length cloning (reply: 1)
1582. Cloning PCR product - double bands or heterozygote (reply: 5)
1583. Problem with BsaB1 digestion - (reply: 1)
1584. vector map - (reply: 4)
1585. problem with ligation - ligation (reply: 6)
1586. Adeno-X Expression System problems - (reply: 3)
1587. purification by gel? - after a first round of PCR (reply: 2)
1588. Experience with NheI, AsiSI, FseI and MluI? - (reply: 4)
1589. Reverse transcriptase PCR - RTPCT (reply: 2)
1590. False positive for colony PCR - (reply: 3)
1562. Can we purify Miniprep DNA and use for transfection? - (reply: 3)
1563. supercoiled control - (reply: 5)
1564. ligation to transformation - (reply: 1)
1565. Digesting PCR products and cleaning up digestions - a few quick questions (reply: 4)
1566. coagulation in SDSPAGE Sample buffer - SDS PAGE (reply: 1)
1567. Hybridation vs Normal PCR - (reply: 2)
1568. Duration of cell line transfected - (reply: 2)
1569. Use Zeocin to isolate Zeocin resistant clones - (reply: 2)
1570. Two same oris (origins of replications) in one plasmid - (reply: 1)
1571. Searching for a pQE40 - (reply: 2)
1572. Beta mercaptoethanol? - (reply: 2)
1573. Retroviral Vector won't digest ideas! - (reply: 1)
1574. Site directed mutagenesis GFP to RFP - (reply: 4)
1575. Kozak Sequences and Mammalian Expression Vectors - (reply: 2)
1576. kits for mrna isolation in india - mrna isolation (reply: 1)
1577. My competent cells are misguiding me - (reply: 3)
1578. Vector self ligation? - (reply: 1)
1579. cloning problems - help! (reply: 4)
1580. High Fidelity DNA Polymerase: how many mutations per every kb of amplified DNA o - (reply: 10)
1581. rabbit intestinal cDNA - Full length cloning (reply: 1)
1582. Cloning PCR product - double bands or heterozygote (reply: 5)
1583. Problem with BsaB1 digestion - (reply: 1)
1584. vector map - (reply: 4)
1585. problem with ligation - ligation (reply: 6)
1586. Adeno-X Expression System problems - (reply: 3)
1587. purification by gel? - after a first round of PCR (reply: 2)
1588. Experience with NheI, AsiSI, FseI and MluI? - (reply: 4)
1589. Reverse transcriptase PCR - RTPCT (reply: 2)
1590. False positive for colony PCR - (reply: 3)