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61. Plasmid Isolation - (reply: 1)
62. RT-PCR primer design - RT-PCR primer design...need ur help (reply: 1)
63. HOW to clone some kinases? - clone CDK2,CDK3,MAPK14 from HeLa (reply: 2)
64. problem in cloning, if any one can help me out - (reply: 2)
65. Transformation failure - (reply: 1)
66. Too many colonies - Too many colonies (reply: 1)
67. Sequential digestion protocol - (reply: 3)
68. TOPO® TA cloning - (reply: 3)
69. non dephosphorylated vector gave white colonies - (reply: 2)
70. Subcloning and Mutations - (reply: 1)
71. shRNA ligation problem - (reply: 3)
72. terrific broth mistake? - (reply: 1)
73. pUC19 ligation - (reply: 1)
74. Ligation problems... - (reply: 2)
75. How to transform with topo vector - (reply: 6)
76. Separating a 2750 base DNA band from a 2600 base DNA band - (reply: 8)
77. Troubleshooting help for PCR - No amplicon: 500bp (reply: 3)
78. Problems with Mutations of 2.1 kb Gene - (reply: 7)
79. In frame clonning adding a restriction site - (reply: 1)
80. PCR bands - why not straight ?&%#@&& - (reply: 3)
81. Help with RT-PCR of 1.4Kb fragment - (reply: 2)
82. Basic of the basic query! - (reply: 3)
83. Restriction enzymes not cutting - (reply: 4)
84. Why use 200 units of ligase? - I am going cuckoo lol (reply: 11)
85. blunt ligation of large fragment - (reply: 6)
86. Yeast Transformation Problems - (reply: 1)
87. Standard sequencing primers - how to find which sequencing primers bind to my DNA? (reply: 2)
88. mRNA concentration - how to concentrate mRNA (reply: 2)
89. ligase temperature - (reply: 5)
90. My clones disappear after subculturing! - (reply: 3)
62. RT-PCR primer design - RT-PCR primer design...need ur help (reply: 1)
63. HOW to clone some kinases? - clone CDK2,CDK3,MAPK14 from HeLa (reply: 2)
64. problem in cloning, if any one can help me out - (reply: 2)
65. Transformation failure - (reply: 1)
66. Too many colonies - Too many colonies (reply: 1)
67. Sequential digestion protocol - (reply: 3)
68. TOPO® TA cloning - (reply: 3)
69. non dephosphorylated vector gave white colonies - (reply: 2)
70. Subcloning and Mutations - (reply: 1)
71. shRNA ligation problem - (reply: 3)
72. terrific broth mistake? - (reply: 1)
73. pUC19 ligation - (reply: 1)
74. Ligation problems... - (reply: 2)
75. How to transform with topo vector - (reply: 6)
76. Separating a 2750 base DNA band from a 2600 base DNA band - (reply: 8)
77. Troubleshooting help for PCR - No amplicon: 500bp (reply: 3)
78. Problems with Mutations of 2.1 kb Gene - (reply: 7)
79. In frame clonning adding a restriction site - (reply: 1)
80. PCR bands - why not straight ?&%#@&& - (reply: 3)
81. Help with RT-PCR of 1.4Kb fragment - (reply: 2)
82. Basic of the basic query! - (reply: 3)
83. Restriction enzymes not cutting - (reply: 4)
84. Why use 200 units of ligase? - I am going cuckoo lol (reply: 11)
85. blunt ligation of large fragment - (reply: 6)
86. Yeast Transformation Problems - (reply: 1)
87. Standard sequencing primers - how to find which sequencing primers bind to my DNA? (reply: 2)
88. mRNA concentration - how to concentrate mRNA (reply: 2)
89. ligase temperature - (reply: 5)
90. My clones disappear after subculturing! - (reply: 3)