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Molecular-Cloning
91.
Ligation problems... -
(reply: 2)
92.
How to transform with topo vector -
(reply: 6)
93.
Separating a 2750 base DNA band from a 2600 base DNA band -
(reply: 8)
94.
Troubleshooting help for PCR - No amplicon: 500bp
(reply: 3)
95.
Problems with Mutations of 2.1 kb Gene -
(reply: 7)
96.
In frame clonning adding a restriction site -
(reply: 1)
97.
PCR bands - why not straight ?&%#@&& -
(reply: 3)
98.
Help with RT-PCR of 1.4Kb fragment -
(reply: 2)
99.
Basic of the basic query! -
(reply: 3)
100.
Restriction enzymes not cutting -
(reply: 4)
101.
Why use 200 units of ligase? - I am going cuckoo lol
(reply: 11)
102.
blunt ligation of large fragment -
(reply: 6)
103.
Yeast Transformation Problems -
(reply: 1)
104.
Standard sequencing primers - how to find which sequencing primers bind to my DNA?
(reply: 2)
105.
mRNA concentration - how to concentrate mRNA
(reply: 2)
106.
ligase temperature -
(reply: 5)
107.
My clones disappear after subculturing! -
(reply: 3)
108.
Mysterious Band in NdeI/BamHI Double Digest -
(reply: 7)
109.
gDNA extraction from mouse ear for PCR - "Hot Shot" extraction
(reply: 1)
110.
Random Library Cloning -
(reply: 1)
111.
I did something stupid - 10 mM dNTPs vs 2.5 mM dNTPs
(reply: 2)
112.
DNA cloning problem -
(reply: 3)
113.
Cloning of plasmid -
(reply: 3)
114.
Expression vector and Stop codon consideration -
(reply: 2)
115.
Please comment on my cloning strategy -
(reply: 5)
116.
transformation issues with ligation product - a double-digested (HindIII / HpaI) vector won't take up an insert
(reply: 5)
117.
Two CMVmin in same plasmid? -
(reply: 2)
118.
Who has a good protocol to cut a plasmid? -
(reply: 1)
119.
it worked! hahahahaha -
(reply: 6)
120.
Problem with Ligation Controls HELP PLEASE - EcoR1 cut site on pEGFPC2 vector will not religate?
(reply: 8)
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