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91. Ligation problems... - (reply: 2)
92. How to transform with topo vector - (reply: 6)
93. Separating a 2750 base DNA band from a 2600 base DNA band - (reply: 8)
94. Troubleshooting help for PCR - No amplicon: 500bp (reply: 3)
95. Problems with Mutations of 2.1 kb Gene - (reply: 7)
96. In frame clonning adding a restriction site - (reply: 1)
97. PCR bands - why not straight ?&%#@&& - (reply: 3)
98. Help with RT-PCR of 1.4Kb fragment - (reply: 2)
99. Basic of the basic query! - (reply: 3)
100. Restriction enzymes not cutting - (reply: 4)
101. Why use 200 units of ligase? - I am going cuckoo lol (reply: 11)
102. blunt ligation of large fragment - (reply: 6)
103. Yeast Transformation Problems - (reply: 1)
104. Standard sequencing primers - how to find which sequencing primers bind to my DNA? (reply: 2)
105. mRNA concentration - how to concentrate mRNA (reply: 2)
106. ligase temperature - (reply: 5)
107. My clones disappear after subculturing! - (reply: 3)
108. Mysterious Band in NdeI/BamHI Double Digest - (reply: 7)
109. gDNA extraction from mouse ear for PCR - "Hot Shot" extraction (reply: 1)
110. Random Library Cloning - (reply: 1)
111. I did something stupid - 10 mM dNTPs vs 2.5 mM dNTPs (reply: 2)
112. DNA cloning problem - (reply: 3)
113. Cloning of plasmid - (reply: 3)
114. Expression vector and Stop codon consideration - (reply: 2)
115. Please comment on my cloning strategy - (reply: 5)
116. transformation issues with ligation product - a double-digested (HindIII / HpaI) vector won't take up an insert (reply: 5)
117. Two CMVmin in same plasmid? - (reply: 2)
118. Who has a good protocol to cut a plasmid? - (reply: 1)
119. it worked! hahahahaha - (reply: 6)
120. Problem with Ligation Controls HELP PLEASE - EcoR1 cut site on pEGFPC2 vector will not religate? (reply: 8)
92. How to transform with topo vector - (reply: 6)
93. Separating a 2750 base DNA band from a 2600 base DNA band - (reply: 8)
94. Troubleshooting help for PCR - No amplicon: 500bp (reply: 3)
95. Problems with Mutations of 2.1 kb Gene - (reply: 7)
96. In frame clonning adding a restriction site - (reply: 1)
97. PCR bands - why not straight ?&%#@&& - (reply: 3)
98. Help with RT-PCR of 1.4Kb fragment - (reply: 2)
99. Basic of the basic query! - (reply: 3)
100. Restriction enzymes not cutting - (reply: 4)
101. Why use 200 units of ligase? - I am going cuckoo lol (reply: 11)
102. blunt ligation of large fragment - (reply: 6)
103. Yeast Transformation Problems - (reply: 1)
104. Standard sequencing primers - how to find which sequencing primers bind to my DNA? (reply: 2)
105. mRNA concentration - how to concentrate mRNA (reply: 2)
106. ligase temperature - (reply: 5)
107. My clones disappear after subculturing! - (reply: 3)
108. Mysterious Band in NdeI/BamHI Double Digest - (reply: 7)
109. gDNA extraction from mouse ear for PCR - "Hot Shot" extraction (reply: 1)
110. Random Library Cloning - (reply: 1)
111. I did something stupid - 10 mM dNTPs vs 2.5 mM dNTPs (reply: 2)
112. DNA cloning problem - (reply: 3)
113. Cloning of plasmid - (reply: 3)
114. Expression vector and Stop codon consideration - (reply: 2)
115. Please comment on my cloning strategy - (reply: 5)
116. transformation issues with ligation product - a double-digested (HindIII / HpaI) vector won't take up an insert (reply: 5)
117. Two CMVmin in same plasmid? - (reply: 2)
118. Who has a good protocol to cut a plasmid? - (reply: 1)
119. it worked! hahahahaha - (reply: 6)
120. Problem with Ligation Controls HELP PLEASE - EcoR1 cut site on pEGFPC2 vector will not religate? (reply: 8)