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661. Can't ligate 2 linear fragments with cohesive ends - (reply: 5)
662. pBJ5 sequence - (reply: 1)
663. Ligation of pBBR with PCR fragments doesn't work - (reply: 3)
664. How to get rid of satellite colonies? - (reply: 17)
665. NsiI digestion problems - NsiI is not working properly? (reply: 1)
666. Confused about cre-lox recombination system - Why is CreatorTM DNA cloning better over other cre-lox systems? (reply: 1)
667. kozak sequence has to be in frame? - (reply: 9)
668. does the high pH of milli Q water will affect my ligation? - (reply: 8)
669. Dilute re-ligation - deletion construct (reply: 1)
670. Weird thing: Cml resistant S. aureus strain (RN4220) - (reply: 1)
671. Problems with cloning - (reply: 4)
672. Issues with pcDNA3.1Directional cloning - (reply: 2)
673. problem with restriction digestion - (reply: 3)
674. Please save me ! - pAdeasy adenovirus vector system (reply: 1)
675. Flag Tagging a PCR product - (reply: 2)
676. Forgot to inactivate SAP (shrimp alkaline phosphatase) - (reply: 6)
677. screening recombinants - (reply: 4)
678. E.coli + pCAMBIA1302 - everything is more complicated than expected... (reply: 4)
679. screening recombinant colonies - (reply: 4)
680. PCR and Digest cleanup before ligation. - A shortcut by avoiding gel purification. (reply: 15)
681. Amplify RANKL for cloning - (reply: 3)
682. Protocol for amplification of gene in plasmid directly from transformed e. coli? - (reply: 2)
683. Introduce restriction sites with PCR primers - (reply: 2)
684. problems in ligation/transformation - (reply: 12)
685. unsuccessful gel purification - (reply: 8)
686. wrong sequencing of T clones - (reply: 3)
687. Gateway Recombination Problems - (reply: 6)
688. Cloning with linkers? - (reply: 5)
689. ligation ratio - (reply: 1)
690. Blunt end cloning PCR product - (reply: 6)
662. pBJ5 sequence - (reply: 1)
663. Ligation of pBBR with PCR fragments doesn't work - (reply: 3)
664. How to get rid of satellite colonies? - (reply: 17)
665. NsiI digestion problems - NsiI is not working properly? (reply: 1)
666. Confused about cre-lox recombination system - Why is CreatorTM DNA cloning better over other cre-lox systems? (reply: 1)
667. kozak sequence has to be in frame? - (reply: 9)
668. does the high pH of milli Q water will affect my ligation? - (reply: 8)
669. Dilute re-ligation - deletion construct (reply: 1)
670. Weird thing: Cml resistant S. aureus strain (RN4220) - (reply: 1)
671. Problems with cloning - (reply: 4)
672. Issues with pcDNA3.1Directional cloning - (reply: 2)
673. problem with restriction digestion - (reply: 3)
674. Please save me ! - pAdeasy adenovirus vector system (reply: 1)
675. Flag Tagging a PCR product - (reply: 2)
676. Forgot to inactivate SAP (shrimp alkaline phosphatase) - (reply: 6)
677. screening recombinants - (reply: 4)
678. E.coli + pCAMBIA1302 - everything is more complicated than expected... (reply: 4)
679. screening recombinant colonies - (reply: 4)
680. PCR and Digest cleanup before ligation. - A shortcut by avoiding gel purification. (reply: 15)
681. Amplify RANKL for cloning - (reply: 3)
682. Protocol for amplification of gene in plasmid directly from transformed e. coli? - (reply: 2)
683. Introduce restriction sites with PCR primers - (reply: 2)
684. problems in ligation/transformation - (reply: 12)
685. unsuccessful gel purification - (reply: 8)
686. wrong sequencing of T clones - (reply: 3)
687. Gateway Recombination Problems - (reply: 6)
688. Cloning with linkers? - (reply: 5)
689. ligation ratio - (reply: 1)
690. Blunt end cloning PCR product - (reply: 6)